Biomaterial Database

Specific fibrinogen quantitation by electroimmunodiffusion in agarose gel containing heparin. 1984

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

In the determination of plasma fibrinogen by electroimmunodiffusion, commercial sodium heparin, previously included in the agarose gel, by interacting with fibrinogen molecules, enhances their anodic mobility more strongly than after carbamylation with potassium cyanate. The proposed method provides an increase in sensitivity and specificity which practically eliminates the influence of fibrinogen degradation products.

UI	MeSH Term	Description	Entries
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003485	Cyanates	Organic salts of cyanic acid containing the -OCN radical.	Cyanate
D004587	Electrophoresis, Agar Gel	Electrophoresis in which agar or agarose gel is used as the diffusion medium.	Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D005340	Fibrinogen	Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.	Coagulation Factor I,Factor I,Blood Coagulation Factor I,gamma-Fibrinogen,Factor I, Coagulation,gamma Fibrinogen
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006493	Heparin	A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.	Heparinic Acid,alpha-Heparin,Heparin Sodium,Liquaemin,Sodium Heparin,Unfractionated Heparin,Heparin, Sodium,Heparin, Unfractionated,alpha Heparin
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia

Related Publications

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

Immunoelectrophoretic quantitation of antithrombin III. Electroimmunodiffusion in agarose gels containing heparin.

January 1977, International journal of peptide and protein research,

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

Rapid quantitation of the immunopreciptin reaction in agarose gel by electroimmunodiffusion with paper discs.

October 1973, Journal of immunological methods,

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

Quantitation of immunoglobulin G by electrophoresis in agarose gel containing antibodies.

November 1970, Scandinavian journal of clinical and laboratory investigation,

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

Quantitation of the fourth complement component by electrophoresis in agarose gel containing antibodies.

September 1970, Clinical and experimental immunology,

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

Determination of amniotic fluid IgM and IgA by hydroxyethylcellulose-agarose gel electroimmunodiffusion.

January 1983, American journal of reproductive immunology : AJRI : official journal of the American Society for the Immunology of Reproduction and the International Coordination Committee for Immunology of Reproduction,

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

Electroblotting of proteins on agarose gel containing specific antibodies.

January 1993, Electrophoresis,

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

Quantitation of the -lipoprotein complex by agarose gel electrophoresis.

July 1971, Clinica chimica acta; international journal of clinical chemistry,

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

Quantitation of the -lipoprotein complex by agarose gel electrophoresis.

January 1973, Clinica chimica acta; international journal of clinical chemistry,

P Fabrizi, and A Provvedi, and C Moauro, and P Tarli

Identification and quantitation of heparins by microelectrophoresis on agarose gel.

March 1973, Analytical biochemistry,