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Preconditioning of Trichinella spiralis nativa larvae in musculature to low temperatures. 1984

H J Smith

Preconditioning of a Trichinella spiralis nativa isolate in ferret and fox musculature was carried out by freezing at -15 degrees C from 0 to 322 days prior to low temperature refrigeration at -32 degrees C. A limited number of preconditioned samples of infected fox musculature was also refrigerated at -45 degrees C. Preconditioned larvae were appreciably more resistant than those subjected to the low temperatures directly. Under the conditions of this investigation, the longer the period of preconditioning, the greater the resistance, (i.e., survival of larvae) observed. The larvae in fox musculature were slightly more resistant than those in ferret musculature. Limited infectivity trials indicated that pre-conditioned larvae surviving low temperature refrigeration of -32 degrees C -45 degrees C retained their infectivity for at least 44 and 37 days, respectively.

UI MeSH Term Description Entries
D007814 Larva Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals. Maggots,Tadpoles,Larvae,Maggot,Tadpole
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D010434 Pepsin A Formed from pig pepsinogen by cleavage of one peptide bond. The enzyme is a single polypeptide chain and is inhibited by methyl 2-diaazoacetamidohexanoate. It cleaves peptides preferentially at the carbonyl linkages of phenylalanine or leucine and acts as the principal digestive enzyme of gastric juice. Pepsin,Pepsin 1,Pepsin 3
D012034 Refrigeration The mechanical process of cooling. Cold Chain,Chain, Cold,Chains, Cold,Cold Chains
D003080 Cold Temperature An absence of warmth or heat or a temperature notably below an accustomed norm. Cold,Cold Temperatures,Temperature, Cold,Temperatures, Cold
D005289 Ferrets Semidomesticated variety of European polecat much used for hunting RODENTS and/or RABBITS and as a laboratory animal. It is in the subfamily Mustelinae, family MUSTELIDAE. Domestic Polecat,Domestic Polecats,European Polecat,European Polecats,Ferret,Mustela putorius,Mustela putorius furo,Polecat, Domestic,Polecat, European,Polecats, Domestic,Polecats, European
D005589 Foxes Any of several carnivores in the family CANIDAE, that possess erect ears and long bushy tails and are smaller than WOLVES. They are classified in several genera and found on all continents except Antarctica. Alopex,Arctic Fox,Pseudalopex,Red Fox,Urocyon,Vulpes,Vulpes vulpes,Fox, Arctic,Fox, Red
D000222 Adaptation, Physiological The non-genetic biological changes of an organism in response to challenges in its ENVIRONMENT. Adaptation, Physiologic,Adaptations, Physiologic,Adaptations, Physiological,Adaptive Plasticity,Phenotypic Plasticity,Physiological Adaptation,Physiologic Adaptation,Physiologic Adaptations,Physiological Adaptations,Plasticity, Adaptive,Plasticity, Phenotypic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014234 Trichinella A genus of parasitic nematodes that causes TRICHINELLOSIS in man and other animal. Trichina,Trichinas,Trichinellas

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