Biomaterial Database

[Congenital angular deformity of the tibia in chick embryo induced by ethane-1-hydroxy-1, 1-diphosphonate (EHDP)]. 1984

Y Morio

Congenital angular deformity of the tibia (CADT) is one of the classic inborn errors. The treatment of the disease is very difficult because of insufficient clinical and experimental data and lack of complete experimental model of the disease. The present study is designed to establish a new experimental model of CADT. In vivo experiment: Only one injection of ethane-1-hydroxy-1,1-diphosphonate (EHDP) (4 X 10(-2)-4 X 10(-1) mumol/g egg wt.) was given into the yolk sac of fertile white leghorn egg at 8 days of incubation. The skeletal deformities of the chick embryos were examined during the period of EHDP-administration until hatching. Furthermore, the effect of EHDP was compared with that of its analogs (dichloromethylene diphosphonate: Cl2MDP and inorganic pyrophosphate: PPi) in a similar manner. In vitro experiment: Tibiae from 9-day-old chick embryos were cultured at 37 degrees C for 6 days by roller-tube method in the medium containing EHDP, Cl2MDP or PPi at concentrations of 4-400 microM to measure dry weight and calcium content of the tibiae. The results are summarized as follows. Angular deformity of the tibia in chick embryo was produced in vivo specifically by EHDP-administration compared to its analogs. There existed the critical stage of bone development and the critical dose of EHDP-administration to induce angular deformity of the tibia. The incidence and the severity of the tibial bowing depended on the dose of EHDP-administration. The thinning of periosteal bone collar and retardation of primary bone marrow formation were observed in EHDP-administered tibia. According to 3H-thymidine autoradiographic study, EHDP inhibited DNA synthesis of osteoblast by about 58% after 2 days of administration. The DNA synthesis of chondrocyte was also inhibited by about 20% after 2 days of EHDP-administration. EHDP had a more inhibitory effect on calcification of chick embryonic tibiae than Cl2MDP and PPi at concentration of 40 microM without influencing the dry weight of tibiae in vitro. This new experimental model of CADT offers a significant possibility to elucidate the etiology of this disease.

UI	MeSH Term	Description	Entries
D011859	Radiography	Examination of any part of the body for diagnostic purposes by means of X-RAYS or GAMMA RAYS, recording the image on a sensitized surface (such as photographic film).	Radiology, Diagnostic X-Ray,Roentgenography,X-Ray, Diagnostic,Diagnostic X-Ray,Diagnostic X-Ray Radiology,X-Ray Radiology, Diagnostic,Diagnostic X Ray,Diagnostic X Ray Radiology,Diagnostic X-Rays,Radiology, Diagnostic X Ray,X Ray Radiology, Diagnostic,X Ray, Diagnostic,X-Rays, Diagnostic
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D004195	Disease Models, Animal	Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases.	Animal Disease Model,Animal Disease Models,Disease Model, Animal
D000014	Abnormalities, Drug-Induced	Congenital abnormalities caused by medicinal substances or drugs of abuse given to or taken by the mother, or to which she is inadvertently exposed during the manufacture of such substances. The concept excludes abnormalities resulting from exposure to non-medicinal chemicals in the environment.	Drug-Induced Abnormalities,Abnormalities, Drug Induced,Abnormality, Drug-Induced,Drug Induced Abnormalities,Drug-Induced Abnormality
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012968	Etidronic Acid	A diphosphonate which affects calcium metabolism. It inhibits ectopic calcification and slows down bone resorption and bone turnover.	EHDP,Ethanehydroxydiphosphonate,Etidronate,Etidronate Disodium,Sodium Etidronate,(1-hydroxyethylene)diphosphonic acid,(1-hydroxyethylene)diphosphonic acid, Tetrapotassium Salt,1,1-hydroxyethylenediphosphonate,1-Hydroxyethane-1,1-Diphosphonate,1-Hydroxyethylidene-1,1-Bisphosphonate,Dicalcium EHDP,Dicalcium Etidronate,Didronel,Disodium 1-Hydroxyethylene Diphosphonate,Disodium Etidronate,Ethanehydroxyphosphate,Etidronate, Tetrapotassium Salt,HEDP,HEDSPA,Hydroxyethanediphosphonate,Hydroxyethylidene Diphosphonic Acid,Phosphonic acid, (1-hydroxyethylidene)bis-, disodium salt,Xidifon,Xidiphon,Xydiphone,1 Hydroxyethane 1,1 Diphosphonate,1 Hydroxyethylidene 1,1 Bisphosphonate,1,1 hydroxyethylenediphosphonate,1-Hydroxyethylene Diphosphonate, Disodium,Diphosphonate, Disodium 1-Hydroxyethylene,Diphosphonic Acid, Hydroxyethylidene,Disodium 1 Hydroxyethylene Diphosphonate,EHDP, Dicalcium,Etidronate, Dicalcium,Etidronate, Disodium,Etidronate, Sodium,Salt Etidronate, Tetrapotassium,Tetrapotassium Salt Etidronate
D013977	Tibia	The second longest bone of the skeleton. It is located on the medial side of the lower leg, articulating with the FIBULA laterally, the TALUS distally, and the FEMUR proximally.	Tibias