Biomaterial Database

The simultaneous identification of seminal acid phosphatase and phosphoglucomutase by starch gel electrophoresis. 1980

H G Linde, and K E Molnar

Although elevated acid phosphatase (AP) activity in vaginal fluid is a consistent indicator for semen, differentiation between vaginal AP and seminal AP provides a more meaningful result. Detection of seminal AP in mixtures of vaginal AP, feces, and blood is accomplished by starch gel electrophoresis, employing the substrate thymolphthalein monophosphate as a selective visualization agent. Genetic phosphoglucomutase isoenzymes are simultaneously separated by this method and allow differentiation in some semen/vaginal fluid mixtures.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010733	Phosphoglucomutase	An enzyme that catalyzes the conversion of alpha D-glucose 1-phosphate to alpha D-glucose 6-phosphate. EC 5.4.2.2.	Glucose Phosphomutase,Phosphomutase, Glucose
D012007	Rectum	The distal segment of the LARGE INTESTINE, between the SIGMOID COLON and the ANAL CANAL.	Rectums
D001826	Body Fluids	Liquid components of living organisms.	Body Fluid,Fluid, Body,Fluids, Body
D002021	Buffers	A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.	Buffer
D004592	Electrophoresis, Starch Gel	Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium.	Starch Gel Electrophoresis
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D005260	Female		Females
D005554	Forensic Medicine	The application of medical knowledge to questions of law.	Legal Medicine,Medicine, Forensic,Medicine, Legal
D005782	Gels	Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.