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The N protein (pN) specified by bacteriophage lambda is an antitermination factor and is required for phage development. pN can be assayed by making use of the observation that the in vitro synthesis of trp mRNA in a reaction programmed with DNA template from lambda trp transducing phage bearing N- and fed- mutations is pN dependent (Ishii et al., 1980). The assay has been used to purify pN. We have observed that pN forms a complex with E. coli protein(s) and is dissociated in the presence of urea. The complex is not formed in host bacteria bearing the nusA-nusB- mutations. pN is a basic protein and heat-stable. Using these characteristics, we have purified pN to virtual homogeneity as judged by polyacrylamide gel electrophoresis in the presence of SDS. pN is a monomeric protein and its mol. wt. is approx. 14 000. The antiterminating activity of pN appears to be enhanced by complex formation with host-encoded protein(s) depending on the nusA and/or nusB gene function.
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
August 1982,
European journal of biochemistry,
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
August 2003,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
June 1980,
Journal of molecular biology,
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
August 1982,
The Journal of biological chemistry,
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
February 1978,
Journal of virology,
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
April 1981,
Gene,
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
February 1980,
The Journal of biological chemistry,
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
October 1985,
Virology,
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
December 1986,
Molecular & general genetics : MGG,
S Ishii, and
K Kuroki, and
Y Sugino, and
F Imamoto
January 1970,
Virology,
