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Peritoneal fluid formation and composition is under the dependence of the ovarian function. Collection of these fluids was performed by laparoscopy in normal cycling women. After absorption of the steroids, they were tested on monolayer pituitary cell cultures in order to study the influence on gonadotrophin release. LH secretion was stimulated through cell the menstrual cycle without any cycling variations. FSH secretion was also increased, excepted in the immediate post-ovulation period. Thus the Peritoneal fluid contains, as follicular fluid, substances with gonadocrinin- and inhibin-like activity. Gonadocrinin activity was not correlated with estradiol and progesterone levels in serum and peritoneal fluid.
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