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1. Hyaluronate extracted from rooster comb was digested by a mixture of beta-N-acetylhexosaminidase and beta-glucuronidase with simultaneous dialysis for 96 h. 2. The produjct, yielding 99.6% of a mixture of mono- and oligo-saccharides, was purified by gel chromatography and analysed for glucuronic acid, N-acetylglucosamine and other sugars. 3. The oligosaccharide portion was chromatographed on DEAE-cellulose, and the effluent fractions were analysed for glucuronic acid and N-acetylglucosamine, reduced with NaBH4, digested by beta-N-acetylhexosaminidase and subjected to acid hydrolysis and glucosamine determination. 4. GlcNAc-GlcA-GlcNAc, GlcA-GlcA-GlcNAc and GlcA-GlcA-GlcA-GlcNAc were the oligosaccharides obtained, which resulted from the transferase activity of the enzymes and represented 57% of the digestion products. The results demonstrate that this hyaluronate is an unbranched polymer of approximatey equal amounts of glucuronic acid and N-acetylglucosamine. The data also indicate that if this glycosaminoglycan contains any of the neutral sugars for which it was analysed, their concentration must be less than 0.020% of the sum of the known components.
M O Longas, and
K Meyer
January 1971,
Steroids,
M O Longas, and
K Meyer
January 1981,
The International journal of biochemistry,
M O Longas, and
K Meyer
September 1967,
Biochemistry,
M O Longas, and
K Meyer
December 1973,
Biochimica et biophysica acta,
M O Longas, and
K Meyer
June 2001,
Journal of clinical microbiology,
M O Longas, and
K Meyer
May 2012,
Bulletin of experimental biology and medicine,
M O Longas, and
K Meyer
January 1978,
Methods in enzymology,
M O Longas, and
K Meyer
September 1967,
Biochemistry,
M O Longas, and
K Meyer
December 1981,
The Journal of biological chemistry,
M O Longas, and
K Meyer
October 1973,
Biochemical and biophysical research communications,
