Biomaterial Database

sn-Glycerol-3-phosphate acyltransferase in a particulate fraction from maturing safflower seeds. 1984

K Ichihara

The properties of the acyl-CoA:sn-glycerol-3-phosphate O-acyltransferase in a 20,000g particulate fraction from maturing safflower seeds were investigated. The optimum pH of the reaction was 7.2. The apparent Km for glycerophosphate was 0.54 mM. Only monoacylglycerophosphate was accumulated in the particulate fraction under normal conditions. Position 1 of glycerophosphate was exclusively esterified with either palmitoyl-CoA or linoleoyl-CoA as acyl donor, while 2-acylglycerophosphate was the minor product. The specificity and selectivity of the acyltransferase for acyl-CoA were broad and somewhat affected by temperature. The concentration of glycerophosphate did not affect the selectivity. These observations suggested that the fatty acid composition of position 1 of safflower triacylglycerol must primarily depend on the composition of the acyl-CoA pool in the site of synthesis, and that growth temperature and the acyl-CoA selectivity of the glycerophosphate acyltransferase may be rather minor factors regarding regulation of the fatty acid composition of position 1 in triacylglycerol.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D002621	Chemistry	A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
D005992	Glycerol-3-Phosphate O-Acyltransferase	An enzyme that transfers acyl groups from acyl-CoA to glycerol-3-phosphate to form monoglyceride phosphates. It acts only with CoA derivatives of fatty acids of chain length above C-10. Also forms diglyceride phosphates. EC 2.3.1.15.	Glycerolphosphate Acyltransferase,Stearyl-CoA Glycerophosphate Transstearylase,Acyl-CoA Sn-Glycerol-3-Phosphate-O-Acyltransferase,Glycerophosphate Acyltransferase,Acyl CoA Sn Glycerol 3 Phosphate O Acyltransferase,Acyltransferase, Glycerolphosphate,Acyltransferase, Glycerophosphate,Glycerol 3 Phosphate O Acyltransferase,Glycerophosphate Transstearylase, Stearyl-CoA,O-Acyltransferase, Glycerol-3-Phosphate,Sn-Glycerol-3-Phosphate-O-Acyltransferase, Acyl-CoA,Stearyl CoA Glycerophosphate Transstearylase,Transstearylase, Stearyl-CoA Glycerophosphate
D000214	Acyl Coenzyme A	S-Acyl coenzyme A. Fatty acid coenzyme A derivatives that are involved in the biosynthesis and oxidation of fatty acids as well as in ceramide formation.	Acyl CoA,Fatty Acyl CoA,Long-Chain Acyl CoA,Acyl CoA, Fatty,Acyl CoA, Long-Chain,CoA, Acyl,CoA, Fatty Acyl,CoA, Long-Chain Acyl,Coenzyme A, Acyl,Long Chain Acyl CoA
D000217	Acyltransferases	Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.	Acyltransferase
D012639	Seeds	The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.	Diaspores,Elaiosomes,Embryos, Plant,Plant Embryos,Plant Zygotes,Zygotes, Plant,Diaspore,Elaiosome,Embryo, Plant,Plant Embryo,Plant Zygote,Seed,Zygote, Plant
D013347	Subcellular Fractions	Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)	Fraction, Subcellular,Fractions, Subcellular,Subcellular Fraction
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures
D055598	Chemical Phenomena	The composition, structure, conformation, and properties of atoms and molecules, and their reaction and interaction processes.	Chemical Concepts,Chemical Processes,Physical Chemistry Concepts,Physical Chemistry Processes,Physicochemical Concepts,Physicochemical Phenomena,Physicochemical Processes,Chemical Phenomenon,Chemical Process,Physical Chemistry Phenomena,Physical Chemistry Process,Physicochemical Phenomenon,Physicochemical Process,Chemical Concept,Chemistry Process, Physical,Chemistry Processes, Physical,Concept, Chemical,Concept, Physical Chemistry,Concept, Physicochemical,Concepts, Chemical,Concepts, Physical Chemistry,Concepts, Physicochemical,Phenomena, Chemical,Phenomena, Physical Chemistry,Phenomena, Physicochemical,Phenomenon, Chemical,Phenomenon, Physicochemical,Physical Chemistry Concept,Physicochemical Concept,Process, Chemical,Process, Physical Chemistry,Process, Physicochemical,Processes, Chemical,Processes, Physical Chemistry,Processes, Physicochemical