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Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in obese animals, but plasma phosphate was unchanged. Plasma CT levels measured by radioimmunoassay (RIA) were significantly decreased in fatty (0.50 +/- 0.03 vs 0.68 +/- 0.03 ng/ml in Leans; P less than 0.001), but thyroidal hormone content was not different between Lean and fatty rats (68.7 +/- 5.1 in Leans vs 60.5 +/- 3.6 ng/gland in fatty rats). mRNA was extracted from 10 thyroids, and translated in a rabbit reticulocyte lysate (NEN) in the presence of [35S]methionine. After polyacrylamide gel electrophoresis, specific immunoprecipitates were autoradiographed and quantified by integration. A 50% decrease in translatable CT mRNA was observed in fatty rats. In basal conditions, the biosynthetic activity of C cells in obese rats correlates with the secretion rate of the hormone in the face of unchanged thyroidal CT contents.
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
October 1988,
Metabolism: clinical and experimental,
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
November 1993,
The Biochemical journal,
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
May 1983,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
July 1983,
Physiology & behavior,
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
September 1985,
The American journal of physiology,
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
August 2005,
Metabolism: clinical and experimental,
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
October 1998,
The American journal of physiology,
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
November 2015,
International journal of obesity (2005),
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
October 1993,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
N Segond, and
A Jullienne, and
E H Tahri, and
J M Garel
March 1992,
Biochimica et biophysica acta,
