Liquid chromatographic determination of sodium salinomycin in feeds, with post-column reaction. 1984

J T Goras, and W R Lacourse

A method for the detection and quantitation of sodium salinomycin in feeds by a liquid chromatographic, post-column reaction system is presented. Sodium salinomycin is leached from feed with hexane, isolated as a dried residue, and dissolved in mobile phase for analysis. The drug is separated from interfering substances on a silica LC column, combined with vanillin reagent in a mixing tee, reacted with vanillin at elevated temperature in a coil, and detected in the effluent stream at 527 nm. Response, as peak area, is linear with concentration. Feed components, other common veterinary drugs, and closely related compounds do not interfere. Average recovery and coefficient of variation (CV) values for liquid spiked feeds and supplements were 101 +/- 3.5% and 100 +/- 0.3%, respectively. Results for laboratory blends and commercial scale feeds were within 6% of intent with CV values ranging from 0.4 to 5.1%.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D011714 Pyrans Pyran
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000821 Animal Feed Foodstuff used especially for domestic and laboratory animals, or livestock. Fodder,Animal Feeds,Feed, Animal,Feeds, Animal,Fodders

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