Biomaterial Database

Liquid chromatographic determination of sodium saccharin, caffeine, aspartame, and sodium benzoate in cola beverages. 1984

T A Tyler

A rapid method has been developed for the determination of sodium saccharin, caffeine, aspartame, and sodium benzoate in cola beverages. The sample is degassed, diluted in water, and injected onto a C18 column. The mobile phase consists of 15% acetonitrile in triethylammonium phosphate buffer adjusted to pH 4.3 with NaOH. The total run time is less than 10 min and the active compounds are determined using absorbance detection at 214 nm.

UI	MeSH Term	Description	Entries
D002021	Buffers	A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.	Buffer
D002110	Caffeine	A methylxanthine naturally occurring in some beverages and also used as a pharmacological agent. Caffeine's most notable pharmacological effect is as a central nervous system stimulant, increasing alertness and producing agitation. It also relaxes SMOOTH MUSCLE, stimulates CARDIAC MUSCLE, stimulates DIURESIS, and appears to be useful in the treatment of some types of headache. Several cellular actions of caffeine have been observed, but it is not entirely clear how each contributes to its pharmacological profile. Among the most important are inhibition of cyclic nucleotide PHOSPHODIESTERASES, antagonism of ADENOSINE RECEPTORS, and modulation of intracellular calcium handling.	1,3,7-Trimethylxanthine,Caffedrine,Coffeinum N,Coffeinum Purrum,Dexitac,Durvitan,No Doz,Percoffedrinol N,Percutaféine,Quick-Pep,Vivarin,Quick Pep,QuickPep
D002853	Chromatography, Liquid	Chromatographic techniques in which the mobile phase is a liquid.	Liquid Chromatography
D005503	Food Additives	Substances used in the processing or storage of foods or animal feed including ANTIOXIDANTS; FOOD PRESERVATIVES; FOOD COLORING AGENTS; FLAVORING AGENTS; ANTI-INFECTIVE AGENTS; EXCIPIENTS and other similarly used substances. Many of the same substances are used as PHARMACEUTIC AIDS.	Additive, Food,Additives, Food,Food Additive
D001218	Aspartame	Flavoring agent sweeter than sugar, metabolized as PHENYLALANINE and ASPARTIC ACID.	Aspartylphenylalanine Methyl Ester,Canderel,Goldswite,Hermesetas Gold,Methyl Aspartylphenylalanine,Milisucre,Nozucar,NutraSweet,SC-18862,Tri-Sweet,Aspartylphenylalanine, Methyl,Gold, Hermesetas,Methyl Ester, Aspartylphenylalanine,SC 18862,SC18862,Tri Sweet,TriSweet
D001565	Benzoates	Derivatives of BENZOIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxybenzene structure.	Benzoate,Benzoic Acids,Acids, Benzoic
D001628	Beverages	Liquids that are suitable for drinking. (From Merriam Webster Collegiate Dictionary, 10th ed)	Beverage
D012439	Saccharin	Flavoring agent and non-nutritive sweetener.	Saccharin Calcium,Saccharin Sodium,Calcium, Saccharin
D019817	Benzoic Acid	A fungistatic compound that is widely used as a food preservative. It is conjugated to GLYCINE in the liver and excreted as hippuric acid.	Potassium Benzoate,Ucephan,Acid, Benzoic,Benzoate, Potassium