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To characterize the specificity of monoclonal antibodies against respiratory syncytial virus, we developed a technique which combined the biotin-avidin system, immunoprecipitation, and transblot electrophoresis. The viral proteins were first biotinylated and then immunoprecipitated with a monoclonal antibody or respiratory syncytial virus immune serum, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and electrophoretically transferred to nitrocellulose paper. The protein bands were located on the paper with avidin-peroxidase plus a precipitable substrate. This procedure gave reproducible results qualitatively similar to those obtained by radioimmunoprecipitation but without the cost, risk of radiation exposure, or disposal problems of radioisotopes. This procedure should have widespread applications.
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
June 1999,
The Pediatric infectious disease journal,
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
October 1983,
Lancet (London, England),
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
June 1989,
Journal of virological methods,
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
July 1989,
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire,
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
January 2023,
Cell host & microbe,
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
May 1984,
Immunology today,
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
August 1998,
Expert opinion on investigational drugs,
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
October 1984,
Anales espanoles de pediatria,
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
September 1993,
Journal of immunological methods,
L J Anderson, and
R A Coombs, and
C Tsou, and
J C Hierholzer
January 2019,
Molekuliarnaia biologiia,
