Biomaterial Database

Semisolid selective-motility enrichment medium for isolation of salmonellae from fecal specimens. 1984

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

A semisolid selective-motility enrichment medium for the isolation of salmonellae from fecal specimens was developed which was based on Rappaport enrichment broth. During a 7-year period more than 30,000 stool samples were tested. The medium showed a high specificity (95.1%) and sensitivity (80.3%) when compared with MacConkey agar, SS agar, and brilliant green agar (after Selenite-F Enrichment [BBL Microbiology Systems]). Furthermore, our isolation rate of Salmonella species from fecal samples showed an increase of 22.3% when this semisolid medium was added to the routine culture media. Growth could easily be interpreted. The medium has a bias toward the isolation of Salmonella paratyphi B, but it is unsatisfactory for detecting the nonmotile strains Salmonella typhi and S. paratyphi A.

UI	MeSH Term	Description	Entries
D009068	Movement	The act, process, or result of passing from one place or position to another. It differs from LOCOMOTION in that locomotion is restricted to the passing of the whole body from one place to another, while movement encompasses both locomotion but also a change of the position of the whole body or any of its parts. Movement may be used with reference to humans, vertebrate and invertebrate animals, and microorganisms. Differentiate also from MOTOR ACTIVITY, movement associated with behavior.	Movements
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012475	Salmonella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species

Related Publications

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

Semisolid blood-free selective-motility medium for the isolation of campylobacters from stool specimens.

May 1989, Journal of clinical microbiology,

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

Secondary selective enrichment of salmonellae from naturally contaminated specimens by using a selective motility system.

April 1979, Applied and environmental microbiology,

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

Comparative studies on enrichment and selective media for isolation of Salmonellae from faecal specimens.

January 1977, Acta veterinaria Scandinavica,

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

Selective-enrichment procedure for isolation of Listeria monocytogenes from fecal and biologic specimens.

May 1986, Applied and environmental microbiology,

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

Improved isolation of salmonellae from faeces using a semisolid Rappaport-Vassiliadis medium.

October 1992, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

Comparison of media for isolation of salmonellae and shigellae from fecal specimens.

July 1971, Applied microbiology,

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

Isolation of Campylobacter spp. from stool specimens with a semisolid medium.

November 1991, Journal of clinical microbiology,

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

ISOLATION OF SALMONELLAE BY SELECTIVE MOTILITY SYSTEMS.

May 1965, Applied microbiology,

H Goossens, and G Wauters, and M de Boeck, and M Janssens, and J P Butzler

A new selective medium for the isolation of salmonellae from stools.

January 1947, American journal of clinical pathology,