[Comparison between cytochemical and gelelectrophoretic demonstration of acid phosphatases from leukocytes (author's transl)]. 1978

H W von Heyden, and R Weber, and W Stein

The cytochemical and gelelectrophoretic demonstration of acid phosphatases with substrates alpha-naphthyl phosphate and naphthol AS-BI phosphate and with couplers fast garnet GBC and hexazotized pararosanilin is described. Cytochemically best results are achieved wit naphthol AS-BI phosphate and pararosanilin, electrophoretically however with alpha-naphthyl phosphate and pararosanilin. This discrepancy ist explained by a different substrate affinity and membrane penetration of substrates.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D007962 Leukocytes White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES). Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D004586 Electrophoresis An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. Electrophoreses
D006651 Histocytochemistry Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods. Cytochemistry
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000135 Acid Phosphatase An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.2. Acid beta-Glycerophosphatase,Acid beta Glycerophosphatase

Related Publications

H W von Heyden, and R Weber, and W Stein
September 1961, Ugeskrift for laeger,
H W von Heyden, and R Weber, and W Stein
December 1979, Nihon Shishubyo Gakkai kaishi,
H W von Heyden, and R Weber, and W Stein
January 1976, Nouvelle revue francaise d'hematologie,
H W von Heyden, and R Weber, and W Stein
January 1978, Praxis und Klinik der Pneumologie,
H W von Heyden, and R Weber, and W Stein
January 1962, Haematologica,
H W von Heyden, and R Weber, and W Stein
January 1974, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
H W von Heyden, and R Weber, and W Stein
January 1990, Folia haematologica (Leipzig, Germany : 1928),
H W von Heyden, and R Weber, and W Stein
April 1978, American journal of veterinary research,
H W von Heyden, and R Weber, and W Stein
January 1973, Zeitschrift fur Anatomie und Entwicklungsgeschichte,
H W von Heyden, and R Weber, and W Stein
January 1973, Zeitschrift fur Anatomie und Entwicklungsgeschichte,
Copied contents to your clipboard!