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Red blood cells from Wistar rats were exposed to milimolar concentrations of t-butyl hydroperoxide. Extensive hemoglobin oxidation (methemoglobin formation), t-butyl hydroperoxide cleavage (t-butanol formation) and peroxidation (measured by oxygen consumption and thiobarbituric acid reactive substances) was observed. Significant chemiluminescence was emitted by the system. Hemoglobin oxidation and t-butanol production were independent of oxygen pressure and free radical scavengers, however, luminescence was enhanced as oxygen pressure increased and it was reduced by addition of free radical scavengers. The spectral distribution of the light emitted suggests that the luminescence detected is not due to singlet oxygen dimol emission. The results are in agreement with a lipid peroxidative mechanism initiated by t-butoxy radicals produced in the interaction of hemoglobin and t-butyl hydroperoxide.
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
July 2005,
Biochemistry. Biokhimiia,
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
January 1996,
International journal of clinical & laboratory research,
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
July 1984,
Biochimica et biophysica acta,
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
January 1996,
International journal of clinical & laboratory research,
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
January 1986,
Cell biochemistry and function,
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
January 2001,
Free radical research,
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
August 1997,
Biochimica et biophysica acta,
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
November 1993,
Biochimica et biophysica acta,
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
January 2006,
Redox report : communications in free radical research,
L A Videla, and
M I Villena, and
G Donoso, and
J de la Fuente, and
E Lissi
September 2016,
Biochemistry and biophysics reports,
