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A method for isolating relatively large quantities of glucose oligomer and polymer subfractions from a corn starch hydrolysate is described. Employing large columns of Bio-Gel P-2 (40-80 microns) at room temperature, we can prepare each day 0.5 to 1.2 grams of oligomeric fractions containing three to four adjacent homologues. The columns are homemade, require no flow adapters, and are operated by gravity elution with water as the solvent. The means for avoiding and overcoming potential difficulties, such as microbial contamination and declining flow rate, are described. With the use of the described method, we can operate a single column continuously for up to twelve months.
H R Sloan, and
B Kerzner, and
C Seckel
June 1997,
Analytical biochemistry,
H R Sloan, and
B Kerzner, and
C Seckel
August 1988,
Analytical biochemistry,
H R Sloan, and
B Kerzner, and
C Seckel
January 1985,
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,
H R Sloan, and
B Kerzner, and
C Seckel
May 2001,
Current protocols in protein science,
H R Sloan, and
B Kerzner, and
C Seckel
November 1994,
Analytical biochemistry,
H R Sloan, and
B Kerzner, and
C Seckel
June 1974,
Carbohydrate research,
H R Sloan, and
B Kerzner, and
C Seckel
January 1982,
Journal of parenteral science and technology : a publication of the Parenteral Drug Association,
H R Sloan, and
B Kerzner, and
C Seckel
December 2008,
Molecular membrane biology,
H R Sloan, and
B Kerzner, and
C Seckel
May 2001,
Current protocols in protein science,
H R Sloan, and
B Kerzner, and
C Seckel
January 2004,
Methods in molecular biology (Clifton, N.J.),
