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The effects of acute cellular or extracellular dehydration on milk intake via suckling were determined in rats 5, 10, 15, and 20 days of age. Milk was made available 10 times to each rat pup by the intravenous infusion of oxytocin to anesthetized test dams. Prior to 15 days of age, milk intake was not affected by either intracellular or extracellular dehydration. In rats 15 days and older, however, both forms of dehydration reduced milk intake at the nipple. Thus, dehydration starts to inhibit suckling behavior at the onset of weaning, when food and water are both taken directly from the environment by the developing animal.
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