Biomaterial Database

[Effect of a short-term protein-free diet on endogenous nitrogen secretion: exocrine pancreas secretion in the pig]. 1984

T Corring, and R Calmes, and A Rérat, and A M Geugneau

A protein-free diet is often used in animal experimentation to estimate the amount of total endogenous nitrogen secreted by the organism. To test the validity of this technique, we carried out a study on pigs describing the short-term (8-day) effect of a protein-free diet on exocrine pancreas nitrogen secretion; this nitrogen was considered as representative of total endogenous nitrogen. After 11 pigs had been adapted to a balanced diet (14% protein), they were chronically fistulated in the pancreatic duct and duodenum. Pancreatic secretion and its various parameters (volume, total protein and chymotrypsin, trypsin, amylase and lipase activities) were measured over a first experimental period of 4 days, during which the pigs continued to receive the balanced diet, and over a second experimental period of 8 days during which they were given a protein-free diet. The results show that the amount of pancreatic protein did not change during the period the protein-free diet was given. After 2 days, chymotrypsin specific activity dropped, while amylase and lipase specific activities decreased little and slowly after 6 days. However, for short-term experiments, the protein-free diet proved to be an adequate technique for determining the production of total endogenous nitrogen in the digestion tract.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009584	Nitrogen	An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
D010179	Pancreas	A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D004044	Dietary Proteins	Proteins obtained from foods. They are the main source of the ESSENTIAL AMINO ACIDS.	Proteins, Dietary,Dietary Protein,Protein, Dietary
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor