BIOMAT Database Logo BIOMAT Database Logo

Affinity chromatography of phenylalanine hydroxylase. The structure of a pteridine adsorbent. 1978

R G Cotton, and I G Jennings

1. Four independent methods have established that the structure of a previously reported pteridine affinity adsorbent, 6,7-dimethyl-5,6,7,8-tetrahydropterin--CH-Sepharose, is 5(CH-Sepharosyl)-6,7-dimethyl-5,6,7,8-tetrahydropterin. 2. A novel reaction, the carbodiimide-promoted coupling of a carboxyl group to N-5 of a tetrahydropterin, is described. 3. Two novel adsorbents, 5-formyl-tetrahydrofolate--AH-Sepharose and 5-methyl-tetrahydrofolate--AH-Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase but also in the study of folate-metabolizing enzymes.

UI MeSH Term Description Entries
D010651 Phenylalanine Hydroxylase An enzyme of the oxidoreductase class that catalyzes the formation of L-TYROSINE, dihydrobiopterin, and water from L-PHENYLALANINE, tetrahydrobiopterin, and oxygen. Deficiency of this enzyme may cause PHENYLKETONURIAS and PHENYLKETONURIA, MATERNAL. EC 1.14.16.1. Phenylalanine 4-Hydroxylase,Phenylalanine 4-Monooxygenase,4-Hydroxylase, Phenylalanine,4-Monooxygenase, Phenylalanine,Hydroxylase, Phenylalanine,Phenylalanine 4 Hydroxylase,Phenylalanine 4 Monooxygenase
D011621 Pteridines Compounds based on pyrazino[2,3-d]pyrimidine which is a pyrimidine fused to a pyrazine, containing four NITROGEN atoms. 1,3,5,8-Tetraazanaphthalene,Pteridine,Pteridinone,Pyrazino(2,3-d)pyrimidine,Pyrazinopyrimidine,Pyrazinopyrimidines,Pyrimido(4,5-b)pyrazine,Pteridinones
D002846 Chromatography, Affinity A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D000327 Adsorption The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily. Adsorptions

Related Publications

R G Cotton, and I G Jennings
A pteridine adsorbent for affinity chromatography.
August 1974, FEBS letters,
R G Cotton, and I G Jennings
A pteridine adsorbent for affinity chromatography.
August 1974, FEBS letters,
R G Cotton, and I G Jennings
Purification of rat liver phenylalanine hydroxylase by affinity chromatography.
April 1980, Archives of biochemistry and biophysics,
R G Cotton, and I G Jennings
Phenylalanine hydroxylase of Macaca irus A simple purification by affinity chromatography.
December 1975, European journal of biochemistry,
R G Cotton, and I G Jennings
Pteridine cofactor of phenylalanine hydroxylase in fetal rat liver.
January 1983, The International journal of biochemistry,
R G Cotton, and I G Jennings
3,4-dihydroxystyrene, a novel microbial inhibitor for phenylalanine hydroxylase and other pteridine-dependent monooxygenases.
September 1984, Biochimica et biophysica acta,
R G Cotton, and I G Jennings
Mutation to phenylalanine of tyrosine 371 in tyrosine hydroxylase increases the affinity for phenylalanine.
November 1998, Biochemistry,
R G Cotton, and I G Jennings
Phenylalanine hydroxylase: function, structure, and regulation.
April 2013, IUBMB life,
R G Cotton, and I G Jennings
Affinity chromatography of Pseudomonas salicylate hydroxylase.
June 1981, Analytical biochemistry,
R G Cotton, and I G Jennings
Determination of phenylalanine hydroxylase activity by liquid chromatography/electrochemistry.
June 1984, Analytical biochemistry,
Copied contents to your clipboard!