Biomaterial Database

Immunodiagnosis of giardiasis by ELISA and studies on cross-reactivity between the anti-Giardia lamblia antibodies and some heterologous parasitic antigens and fractions. 1984

S T Haralabidis

Specific antibodies against Giardia lamblia in 92 sera from asymptomatic carriers were investigated by ELISA. Using cyst antigen, antibodies were detected in 91 of the sera, by means of alkaline phosphatase-labelled antispecies immunoglobulin. The E/405nm values of the positive sera ranged between 0.350 and 1.700, whereas in the 78 control sera they were 0.270 +/- 0.220. Cross-reactivity between the anti-G. lamblia antibodies and 18 heterologous parasitic antigens and fractions was also studied. Cross-reactions were observed with antigens of Toxoplasma gondii, Sarcocystis spp., Leishmania donovani, Fasciola hepatica Y, F. hepatica I, Echinococcus granulosus AM, Cysticercus tenuicollis, Taenia saginata, Dipylidium caninum Y, D. caninum I, Trichuris suis Y, T. suis I, Trichinella spiralis and Litomosoides carinii. No cross-reactions were observed with the antigens and fractions of Entamoeba histolytica, Dicrocoelium dendriticum, Schistosoma mansoni and Echinococcus granulosus Y. The results show that the ELISA could be easily and very successfully applied for routine diagnosis of giardiasis.

UI	MeSH Term	Description	Entries
D010271	Parasites	Invertebrate organisms that live on or in another organism (the host), and benefit at the expense of the other. Traditionally excluded from definition of parasites are pathogenic BACTERIA; FUNGI; VIRUSES; and PLANTS; though they may live parasitically.	Parasite
D003429	Cross Reactions	Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.	Cross Reaction,Reaction, Cross,Reactions, Cross
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005872	Giardia	A genus of flagellate intestinal EUKARYOTES parasitic in various vertebrates, including humans. Characteristics include the presence of four pairs of flagella arising from a complicated system of axonemes and cysts that are ellipsoidal to ovoidal in shape.	Lamblia,Giardias,Lamblias
D005873	Giardiasis	An infection of the SMALL INTESTINE caused by the flagellated protozoan GIARDIA. It is spread via contaminated food and water and by direct person-to-person contact.	Lambliasis,Giardia Infection,Giardia duodenalis Infection,Giardia intestinalis Infection,Giardia lamblia Infection,Giardia Infections,Giardia duodenalis Infections,Giardia intestinalis Infections,Giardia lamblia Infections,Giardiases,Infection, Giardia,Infection, Giardia duodenalis,Infection, Giardia intestinalis,Infection, Giardia lamblia,Infections, Giardia,Infections, Giardia duodenalis,Infections, Giardia intestinalis,Infections, Giardia lamblia,Lambliases
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D000941	Antigens	Substances that are recognized by the immune system and induce an immune reaction.	Antigen