Biomaterial Database

Direct spectrometric determination of serum bile acids in the dog and cat. 1984

S A Center, and C R Leveille, and B H Baldwin, and B C Tennant

Serum bile acid concentrations have been shown to be a predictive indicator of hepatobiliary disease in persons. However, there has been only limited use of bile acid values in the clinical diagnosis of hepatobiliary disease in the dog and cat because of technical difficulties associated with many bile acid assays. A rapid enzymatic method previously developed for the quantitation of 3-hydroxy bile acids in persons has been adapted for use in the dog and cat. Nonsulfated 3-hydroxy bile acids are converted to 3-oxo bile acids by 3 alpha-hydroxysteroid dehydrogenase and reduction of NAD+ to NADH. In a coupled diaphorase catalyzed reaction, H+ is transferred to nitrotetrazolium blue to produce a diformazan dye, which is measured spectrophotometrically at 540 nm. Nonspecific interfering dehydrogenase activities present in the dog and the cat serums were inhibited by heating the serum to 60 C for 30 minutes or by the addition of sodium pyruvate. Standard curves prepared from various serum sodium taurocholate concentrations in dogs and cats are linear to 250 mumol/L. The assay is sensitive for the detection of bile acid concentrations as low as 2.5 mumol/L in sera from dogs and cats. In validation studies quantitative recovery of known concentrations of 7 primary and secondary, conjugated and unconjugated, 3-hydroxy bile acids from pooled canine serum was 95.3 +/- 7.9% (mean +/- SEM) and that from pooled feline serum was 101.4 +/- 8.2%.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D008297	Male		Males
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D004285	Dogs	The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)	Canis familiaris,Dog
D004435	Eating	The consumption of edible substances.	Dietary Intake,Feed Intake,Food Intake,Macronutrient Intake,Micronutrient Intake,Nutrient Intake,Nutritional Intake,Ingestion,Dietary Intakes,Feed Intakes,Intake, Dietary,Intake, Feed,Intake, Food,Intake, Macronutrient,Intake, Micronutrient,Intake, Nutrient,Intake, Nutritional,Macronutrient Intakes,Micronutrient Intakes,Nutrient Intakes,Nutritional Intakes
D005215	Fasting	Abstaining from FOOD.	Hunger Strike,Hunger Strikes,Strike, Hunger,Strikes, Hunger
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001647	Bile Acids and Salts	Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones.	Bile Acid,Bile Salt,Bile Salts,Bile Acids,Acid, Bile,Acids, Bile,Salt, Bile,Salts, Bile
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.