Biomaterial Database

Rapid reversed-phase high-performance liquid chromatographic analysis of steroid products. 1978

C Burgess

The optimisation of conditions for the application of reversed-phase high-performance liquid chromatography (HPLC) to the analysis of steroid products is discussed. The influence of temperature and flow-rate on column efficiency are described. At 60 degrees, the reduced velocity required to give maximum efficiency, is approximately twice that found at room temperature, allowing faster through-put. (hv) curves are reported for 5-micron Spherisorb-ODS and Hypersil-ODS material for corticosteroids at 60 degrees. On-column injection techniques are discussed and data presented, showing that for corticosteroids on-column loop injection can be efficient as septum injection using the techniques described.

UI	MeSH Term	Description	Entries
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002990	Clobetasol	A derivative of PREDNISOLONE with high glucocorticoid activity and low mineralocorticoid activity. Absorbed through the skin faster than FLUOCINONIDE, it is used topically in treatment of PSORIASIS but may cause marked adrenocortical suppression.	Clobetasol 17-Propionate,Clobetasol Propionate,Clobex,Clofenazon,Cormax,Dermovate,Embeline,Embeline E,OLUX,Temovate,Clobetasol 17 Propionate
D004304	Dosage Forms	Completed forms of the pharmaceutical preparation in which prescribed doses of medication are included. They are designed to resist action by gastric fluids, prevent vomiting and nausea, reduce or alleviate the undesirable taste and smells associated with oral administration, achieve a high concentration of drug at target site, or produce a delayed or long-acting drug effect.	Dosage Form,Form, Dosage,Forms, Dosage
D001623	Betamethasone	A glucocorticoid given orally, parenterally, by local injection, by inhalation, or applied topically in the management of various disorders in which corticosteroids are indicated. Its lack of mineralocorticoid properties makes betamethasone particularly suitable for treating cerebral edema and congenital adrenal hyperplasia. (From Martindale, The Extra Pharmacopoeia, 30th ed, p724)	Betadexamethasone,Flubenisolone,Celeston,Celestona,Celestone,Cellestoderm
D001624	Betamethasone Valerate	The 17-valerate derivative of BETAMETHASONE. It has substantial topical anti-inflammatory activity and relatively low systemic anti-inflammatory activity.	Betamethasone 17-Valerate,Flubenisolonvalerate,Betnovate,17-Valerate, Betamethasone,Betamethasone 17 Valerate,Valerate, Betamethasone
D013259	Steroids, Fluorinated	Steroids which are substituted with one or more fluorine atoms in any position.	Fluorinated Steroids