-transparent.png){kind=logo}
Alterations in the surface potential difference (delta U) of asolectin planar bilayer lipid membranes were measured following the adsorption of isolated matrix protein (M-protein) or neuraminidase of influenza virus. The method used was based upon measurement of the bilayer lipid membrane capacitance current second harmonic. The delta U dependence on the M-protein and neuraminidase concentration indicates different mechanisms of adsorption of these viral proteins by the lipid bilayer. The conductance (G0) dependence of the bilayer lipid membrane with different compositions on the concentration of isolated surface glycoproteins, hemagglutinin and neuraminidase, M-protein or neuraminidase was investigated. The change in G0 for M-protein was observed only after adsorption saturation had been achieved. Neuraminidase alone does not affect the membrane conductivity. The surface charge and lipid composition of the lipid bilayer influences the adsorption and incorporation of influenza virus M-protein and surface glycoproteins. The reversibility of protein incorporation into the bilayers was investigated by a perfusion technique. The results show reversibility of surface glycoprotein incorporation while M-protein binding appears to be irreversible.
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
January 1980,
Acta biologica et medica Germanica,
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
September 1981,
Biochemical and biophysical research communications,
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
January 1982,
Biofizika,
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
January 1992,
Methods in enzymology,
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
January 1974,
Methods in enzymology,
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
June 1976,
Biochemical pharmacology,
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
September 1989,
Plant physiology,
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
January 1983,
Biofizika,
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
April 1980,
Biophysical journal,
S El Karadaghi, and
J A Zakomirdin, and
C Shimane, and
D J Bucher, and
V A Tverdislov, and
I G Kharitonenkov
January 1990,
Biofizika,
