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Phospholipase A2 was purified from rat pancreas by heat treatment of the homogenate and the sequential use of DEAE-Sepharose chromatography, CM-Sepharose chromatography, and reverse-phase high-performance liquid chromatography (HPLC). Prophospholipase A2 was not separated from the phospholipase A2 by CM-Sepharose chromatography under the conditions used, but it was well resolved by the reverse-phase HPLC. The enzyme was homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and on analytical HPLC, and its molecular weight was estimated to be 14,000. The enzyme specifically hydrolyzed an acylester bond at the sn-2-position of the phospholipid examined. The purified enzyme has a pH optimum in the range of pH 9.5 to 10.5 and requires the presence of Ca2+ (3 mM) and sodium deoxycholate (0.1%) for optimum activity. The amino acid sequence of the first 32 residues in the N-terminal region of the enzyme was determined. The sequence revealed a marked homology with those of pancreatic phospholipases A2 of man, pig, ox, and horse, and porcine intestinal phospholipase A2 reported previously.
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
February 1993,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
May 1987,
Journal of biochemistry,
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
November 1980,
Biochimica et biophysica acta,
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
October 1983,
Clinical chemistry,
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
February 2004,
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
August 1998,
Journal of biochemistry,
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
June 1992,
Protein expression and purification,
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
July 1990,
Biological chemistry Hoppe-Seyler,
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
September 1988,
Journal of biochemistry,
T Ono, and
H Tojo, and
K Inoue, and
H Kagamiyama, and
T Yamano, and
M Okamoto
November 1994,
Journal of biochemistry,
