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The serotype-specific capsular polysaccharide from two strains of Pasteurella haemolytica serotype A1 organisms was purified and characterized by chemical analysis and NMR spectroscopy. The polymer has the structure----3)-O-(2-acetamido-2-deoxy-4-O-acetyl-beta-D-mannopyranos yluronic acid)-(1----4)-O-(2-acetamido-2-deoxy-beta-D-mannopyranose)-(1----. The polysaccharide was immunogenic (able to evoke production of antibodies) for sheep but not for rabbits. Immuno electron-microscopy studies using the Protein A-gold technique showed the polysaccharide to be peripherally located on the bacterial surface. Reduction, oxidation and de-O-acetylation of the polymer did not appear to alter its immunological precipitability with specific antiserum, but all three treatments destroyed its ability to adhere to sheep erythrocytes at neutral pH. De-N-acetylation of the polymer destroyed both immunological precipitability and erythrocyte adherence.
C Adlam, and
J M Knights, and
A Mugridge, and
J C Lindon, and
P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
February 1993,
Carbohydrate research,
C Adlam, and
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A Mugridge, and
J C Lindon, and
P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
November 1988,
American journal of veterinary research,
C Adlam, and
J M Knights, and
A Mugridge, and
J C Lindon, and
P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
January 1993,
Vaccine,
C Adlam, and
J M Knights, and
A Mugridge, and
J C Lindon, and
P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
September 1986,
Infection and immunity,
C Adlam, and
J M Knights, and
A Mugridge, and
J C Lindon, and
P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
February 1989,
Microbial pathogenesis,
C Adlam, and
J M Knights, and
A Mugridge, and
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P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
March 1996,
Veterinary immunology and immunopathology,
C Adlam, and
J M Knights, and
A Mugridge, and
J C Lindon, and
P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
May 1990,
Veterinary pathology,
C Adlam, and
J M Knights, and
A Mugridge, and
J C Lindon, and
P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
May 1993,
American journal of veterinary research,
C Adlam, and
J M Knights, and
A Mugridge, and
J C Lindon, and
P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
December 1995,
Vaccine,
C Adlam, and
J M Knights, and
A Mugridge, and
J C Lindon, and
P R Baker, and
J E Beesley, and
B Spacey, and
G R Craig, and
L K Nagy
October 1991,
Infection and immunity,
