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Recent work had indicated the presence of a non selenium-dependent glutathione peroxidase activity in rat liver in addition to the selenium-dependent activity. The present study was undertaken to learn whether the glutathione S-transferases are reponsible for the non selenium-dependent glutathione peroxidase activity and to study the effect of selenium deficiency on those enzymes. Glutathione S-transferase B was purified by an established method using carboxymethyl cellulose ion exchange chromatography and studied. It exhibited glutathione peroxidase activity toward cumene hydroperoxide and t-butyl hydroperoxide. A limiting Km of 0.55 mM was determined for cumene hydroperoxide. Sulfobromophthalein was found to be a competitive inhibitor with respect to cumene hydroperoxide of the glutathione peroxidase activity of glutathione S-transferase B. Selenium deficiency caused an increase in glutathione S-transferase activity. These results establish that glutathione S-transferase B contributes to the non selenium-dependent glutathione peroxidase activity in rat liver and show that it increases in selenium deficiency when the selenium-dependent glutathione peroxidase is decreased.
R A Lawrence, and
L K Parkhill, and
R F Burk
August 1985,
Zentralblatt fur Veterinarmedizin. Reihe A,
R A Lawrence, and
L K Parkhill, and
R F Burk
January 1990,
The Journal of biological chemistry,
R A Lawrence, and
L K Parkhill, and
R F Burk
January 1998,
Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer,
R A Lawrence, and
L K Parkhill, and
R F Burk
January 1993,
Biological trace element research,
R A Lawrence, and
L K Parkhill, and
R F Burk
May 1980,
The Proceedings of the Nutrition Society,
R A Lawrence, and
L K Parkhill, and
R F Burk
April 1981,
The Journal of nutrition,
R A Lawrence, and
L K Parkhill, and
R F Burk
April 1985,
Biochemistry international,
R A Lawrence, and
L K Parkhill, and
R F Burk
May 1995,
Clinica chimica acta; international journal of clinical chemistry,
R A Lawrence, and
L K Parkhill, and
R F Burk
May 1987,
The Journal of nutrition,
R A Lawrence, and
L K Parkhill, and
R F Burk
June 1994,
Danish medical bulletin,
