Biomaterial Database

[Mutant gene expression in mouse aggregation chimeras. 3. The brachypodism-H gene]. 1984

N A Malinina, and B N Kindiakov, and B V Koniukhov

The expression of gene brachypodism-H (bpH) was studied on the 12-, 14-, 16-, 17-, 19-day old embryos and 20-day old bpH/bpH C/C----+/+ c + c mice. In 18 chimaeras, the percentage of pigmented cells in the retinal pigment epithelium varied from 2.5 to 84.0%, suggesting a different proportion of the mutant and normal cells in their tissues. The limb development was quite normal in all chimaeras: even in the presence of 84% of mutant cells the lengths of femur, fibula and foot did not differ from those in the control animals. The differentiation and histogenesis of these bones proceeded also quite normally. No increase in cell degeneration was noted and the bpH/bpH cartilage cells were missing, indicating the normalization of the mutant chondrocytes. The absence of the bpH gene expression in the chimaeras appears to be due to a metabolic cooperation between the mutant and normal cells taking place at the early stages of limb development.

UI	MeSH Term	Description	Entries
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D009024	Morphogenesis	The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D010857	Pigment Epithelium of Eye	The layer of pigment-containing epithelial cells in the RETINA; the CILIARY BODY; and the IRIS in the eye.	Eye Pigment Epithelium
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002678	Chimera	An individual that contains cell populations derived from different zygotes.	Hybrids,Chimeras,Hybrid
D005121	Extremities	The farthest or outermost projections of the body, such as the HAND and FOOT.	Limbs,Extremity,Limb
D005260	Female		Females
D005269	Femur	The longest and largest bone of the skeleton, it is situated between the hip and the knee.	Trochanter,Greater Trochanter,Lesser Trochanter,Femurs,Greater Trochanters,Lesser Trochanters,Trochanter, Greater,Trochanter, Lesser,Trochanters,Trochanters, Greater,Trochanters, Lesser
D005360	Fibula	The bone of the lower leg lateral to and smaller than the tibia. In proportion to its length, it is the most slender of the long bones.	Fibulas