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This study describes a method for the isolation of cells from paraffin embedded tissues for DNA-Feulgen-cytophotometric measurements. The relevance of the method used is demonstrated by the analysis of the DNA-distribution pattern of cells isolated from an alveolar sarcoma of the soft tissues of a 10-year old girl. The comparison between freshly prepared imprint preparations and specimens after pepsin--extraction clearly shows, that the preparation mode used did not influence the relative DNA-content. The mean values of DNA/nuclei did not show a decrease even after prolonged pepsin treatment (90 minutes). Therefore this method permits access to stored histopathological material for retrospective DNA-Feulgen-Cytophotometric investigations.
R Delgado, and
G Mikuz, and
F Hofstädter
July 1988,
Morphologiai es igazsagugyi orvosi szemle,
R Delgado, and
G Mikuz, and
F Hofstädter
January 1993,
Methods in molecular biology (Clifton, N.J.),
R Delgado, and
G Mikuz, and
F Hofstädter
June 1980,
Analytical and quantitative cytology,
R Delgado, and
G Mikuz, and
F Hofstädter
March 1992,
American journal of clinical pathology,
R Delgado, and
G Mikuz, and
F Hofstädter
November 1992,
American journal of clinical pathology,
R Delgado, and
G Mikuz, and
F Hofstädter
January 1990,
Methods in cell biology,
R Delgado, and
G Mikuz, and
F Hofstädter
January 1994,
Methods in cell biology,
R Delgado, and
G Mikuz, and
F Hofstädter
January 1990,
Disease markers,
R Delgado, and
G Mikuz, and
F Hofstädter
May 2001,
Current protocols in human genetics,
R Delgado, and
G Mikuz, and
F Hofstädter
February 2009,
Cold Spring Harbor protocols,
