Biomaterial Database

Studies on the enhancement of human eosinophil function by mononuclear cell products in vitro. 1984

M Veith, and D W Taylor, and K Thorne, and B A Richardson, and A E Butterworth

Previous studies have shown that human mononuclear cells produce during culture an activity that enhances the functional properties of mature eosinophils, including their helminthotoxic potential (Veith & Butterworth, 1983). We have now found that the enhancing activity in mononuclear cell culture supernatants (MCS) is stable to dialysis for 24 h at pH 2 and sensitive to trypsin (0.1 mg/ml). It has a principal pI of 4.4 and possible secondary peaks with pI values of 5.0 and 5.7. The effect of MCS on eosinophils appears to be a general early stimulation of several functions. In comparison with eosinophils incubated in medium only, the proportion of eosinophils with Fc receptors is increased after incubation with MCS for 3.5 h (39 +/- 11% vs 16 +/- 3%) and 7 h (28 +/- 4% vs 17 +/- 3%). The temperature-dependent phase of eosinophil adherence to antibody coated schistosomula is enhanced after 3-4 h. The uptake of deoxyglucose is enhanced after a 1 h pre-incubation with MCS (24 +/- 1 X 10(3) d/min), compared with that of controls (15 +/- 2 X 10(3) d/min). Whether these effects are due to one mediator in MCS awaits further investigation.

UI	MeSH Term	Description	Entries
D007525	Isoelectric Focusing	Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.	Electrofocusing,Focusing, Isoelectric
D009000	Monocytes	Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.	Monocyte
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D011961	Receptors, Fc	Molecules found on the surface of some, but not all, B-lymphocytes, T-lymphocytes, and macrophages, which recognize and combine with the Fc (crystallizable) portion of immunoglobulin molecules.	Fc Receptors,Fc Receptor,Receptor, Fc
D002448	Cell Adhesion	Adherence of cells to surfaces or to other cells.	Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002474	Cell-Free System	A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)	Cellfree System,Cell Free System,Cell-Free Systems,Cellfree Systems,System, Cell-Free,System, Cellfree,Systems, Cell-Free,Systems, Cellfree
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003847	Deoxyglucose	2-Deoxy-D-arabino-hexose. An antimetabolite of glucose with antiviral activity.	2-Deoxy-D-glucose,2-Deoxyglucose,2-Desoxy-D-glucose,2 Deoxy D glucose,2 Deoxyglucose,2 Desoxy D glucose
D004804	Eosinophils	Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.	Eosinophil
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man