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Artificial cell-microencapsulated phenylalanine ammonia-lyase. 1984

L Bourget, and T M Chang

Phenylalanine ammonia-lyase (PAL) is immobilized in collodion artificial cells. Once technical problems associated with the encapsulation of this enzyme were solved, the enzyme kinetics were compared to PAL in free solution. Microencapsulated PAL has an apparent enzyme activity that is 20% of the activity of enzyme in free solution. The Km for both free and immobilized PAL is 475 microM. The Vm for the microencapsulated PAL is 9 microM/min, whereas that of PAL in free solution is 55 microM/min.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D010650 Phenylalanine Ammonia-Lyase An enzyme that catalyzes the deamination of PHENYLALANINE to form trans-cinnamate and ammonia. Phenylalanine-Tyrosine Ammonia-lyase,Ammonia-Lyase, Phenylalanine,Ammonia-lyase, Phenylalanine-Tyrosine,Phenylalanine Ammonia Lyase,Phenylalanine Tyrosine Ammonia lyase
D002214 Capsules Hard or soft soluble containers used for the oral administration of medicine. Capsule,Microcapsule,Microcapsules
D004800 Enzymes, Immobilized Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields. Immobilized Enzymes,Enzyme, Immobilized,Immobilized Enzyme
D000642 Ammonia-Lyases Enzymes that catalyze the formation of a carbon-carbon double bond by the elimination of AMMONIA. EC 4.3.1. Ammonia Lyase,Ammonia-Lyase,Ammonia Lyases,Lyase, Ammonia

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