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A cell lysate system by which DNA repair (unscheduled) synthesis induced by DNA damaging agents can be measured at high sensitivity as previously reported (9, 18) was characterized. Time-course experiments in which the in vivo incubation time with hydroxyurea and arabinofuranosyl cytosine after UV irradiation was changed suggested that the number of single strand gaps increased in the presence of these drugs. Alkaline sucrose density gradient analysis of prelabeled DNA revealed that the repair apparatus in the drug-treated cells was not irreversibly impaired. Product DNA in this lysate system was compared with that of an in vitro replication system on alkaline CsC1 equilibrium density gradient, and the results showed that DNA synthesis in the lysate system was "repair-type" synthesis. When the cells were labeled with 5-bromo-2'-deoxyuridine (BrdUrd) in vivo and then labeled with [3H] dTTP in vitro, the photolysis of BrdUrd-labeled DNA decreased the molecular weight, indicating that DNA synthesis in the cell lysate was a continuation of DNA repair in vivo.
K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
March 1969,
Radiation research,
K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
May 2000,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
February 1972,
International journal of radiation biology and related studies in physics, chemistry, and medicine,
K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
January 1980,
Annals of the New York Academy of Sciences,
K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
January 1991,
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K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
January 1989,
Radiation and environmental biophysics,
K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
January 1983,
Environmental mutagenesis,
K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
September 1989,
International journal of radiation biology,
K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
January 2013,
Methods in molecular biology (Clifton, N.J.),
K Yamada, and
F Hanaoka, and
H Kato, and
M Yamada
February 1973,
Archives internationales de physiologie et de biochimie,
