Biomaterial Database

Determination of vitamin A and vitamin A acetate by high-performance liquid chromatography with fluorescence detection. 1984

C A Collins, and C K Chow

A rapid and sensitive high-performance liquid chromatographic method with fluorescence detection has been devised for the analysis of vitamin A (retinol) and its acetate. The method employs a C18 reversed-phase column and methanol as an eluent. The detection of these two compounds is monitored with fluorescence excitation at 348 nm and emission at 470 nm. Detector noise established the lower limit of quantitation at approximately 0.5 ng. Plasma samples were employed to evaluate the accuracy, reproducibility, and applicability of the method. Less than 1 ng of vitamin A in plasma (as low as 1 microliter) can be quantitated by this procedure.

UI	MeSH Term	Description	Entries
D002338	Carotenoids	The general name for a group of fat-soluble pigments found in green, yellow, and leafy vegetables, and yellow fruits. They are aliphatic hydrocarbons containing 4 terpene subunits.	Carotenes,Carotenoid,Tetraterpene Derivatives,Tetraterpenes,Carotene,Derivatives, Tetraterpene
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004224	Diterpenes	Twenty-carbon compounds derived from MEVALONIC ACID or deoxyxylulose phosphate.	Diterpene,Diterpenes, Cembrane,Diterpenes, Labdane,Diterpenoid,Labdane Diterpene,Norditerpene,Norditerpenes,Norditerpenoid,Cembranes,Diterpenoids,Labdanes,Norditerpenoids,Cembrane Diterpenes,Diterpene, Labdane,Labdane Diterpenes
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000084562	Retinyl Esters	A carboxylic ester of retinol formed by condensation of the hydroxy group of retinol with a carboxy group.	All-Trans-Retinyl Ester,All-Trans-Retinyl Esters,Retinyl Ester,All Trans Retinyl Ester,All Trans Retinyl Esters,Ester, All-Trans-Retinyl,Ester, Retinyl
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D014801	Vitamin A	Retinol and derivatives of retinol that play an essential role in metabolic functioning of the retina, the growth of and differentiation of epithelial tissue, the growth of bone, reproduction, and the immune response. Dietary vitamin A is derived from a variety of CAROTENOIDS found in plants. It is enriched in the liver, egg yolks, and the fat component of dairy products.	Retinol,11-cis-Retinol,3,7-dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4,6,8-nonatetraen-1-ol, (all-E)-Isomer,All-Trans-Retinol,Aquasol A,Vitamin A1,All Trans Retinol
D019207	beta Carotene	A carotenoid that is a precursor of VITAMIN A. Beta carotene is administered to reduce the severity of photosensitivity reactions in patients with erythropoietic protoporphyria (PORPHYRIA, ERYTHROPOIETIC).	Betacarotene,BellaCarotin,Carotaben,Max-Caro,Provatene,Solatene,Vetoron,beta-Carotene,Carotene, beta,Max Caro,MaxCaro