BIOMAT Database Logo BIOMAT Database Logo

Influence of molybdate, ionic strength and pH on ligand binding to the glucocorticoid receptor. 1984

P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard

The addition of molybdate to rabbit liver cytosol increased significantly the affinity of the glucocorticoid receptor for [3H] dexamethasone without influencing the concentration of binding sites. This effect was concentration dependent. Analysis of the binding data by curve-fitting and Scatchard plot revealed the occurrence of a complex binding process in the presence of molybdate. The pH-dependence curve of the binding was shifted towards alkaline values by the oxyanion. Taken together, these data suggest that molybdate exerts its effects via an interaction with the receptor molecule.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008982 Molybdenum A metallic element with the atomic symbol Mo, atomic number 42, and atomic weight 95.95. It is an essential trace element, being a component of the enzymes xanthine oxidase, aldehyde oxidase, and nitrate reductase. Molybdenum-98,Molybdenum 98
D009994 Osmolar Concentration The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent. Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D011965 Receptors, Glucocorticoid Cytoplasmic proteins that specifically bind glucocorticoids and mediate their cellular effects. The glucocorticoid receptor-glucocorticoid complex acts in the nucleus to induce transcription of DNA. Glucocorticoids were named for their actions on blood glucose concentration, but they have equally important effects on protein and fat metabolism. Cortisol is the most important example. Corticoid Type II Receptor,Glucocorticoid Receptors,Glucocorticoids Receptor,Corticoid II Receptor,Corticoid Type II Receptors,Glucocorticoid Receptor,Receptors, Corticoid II,Receptors, Corticoid Type II,Receptors, Glucocorticoids,Corticoid II Receptors,Glucocorticoids Receptors,Receptor, Corticoid II,Receptor, Glucocorticoid,Receptor, Glucocorticoids
D011987 Receptors, Steroid Proteins found usually in the cytoplasm or nucleus that specifically bind steroid hormones and trigger changes influencing the behavior of cells. The steroid receptor-steroid hormone complex regulates the transcription of specific genes. Corticosteroid Receptors,Receptors, Corticosteroid,Steroid Receptors,Corticosteroid Receptor,Receptors, Steroids,Steroid Receptor,Receptor, Corticosteroid,Receptor, Steroid,Steroids Receptors
D003600 Cytosol Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components. Cytosols
D003907 Dexamethasone An anti-inflammatory 9-fluoro-glucocorticoid. Hexadecadrol,Decaject,Decaject-L.A.,Decameth,Decaspray,Dexasone,Dexpak,Hexadrol,Maxidex,Methylfluorprednisolone,Millicorten,Oradexon,Decaject L.A.
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations

Related Publications

P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
Molybdate inhibits glucocorticoid-receptor complex binding to RNA.
February 1987, Molecular and cellular endocrinology,
P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
Influence of ionic strength, pH and aptamer configuration for binding affinity to thrombin.
January 2007, Bioelectrochemistry (Amsterdam, Netherlands),
P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
Influence of pH and ionic strength on IgG adsorption to vials.
June 2011, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V,
P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
Effects of pH and ionic strength on the binding of L-tri-iodothyronine to the solubilized nuclear receptor.
December 1985, The Biochemical journal,
P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
The influence of ionic strength, nutrients and pH on bacterial adhesion to metals.
May 2008, Journal of colloid and interface science,
P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
Structural changes in hemoglobin during adsorption to solid surfaces: effects of pH, ionic strength, and ligand binding.
October 1998, Proceedings of the National Academy of Sciences of the United States of America,
P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
Effects of ionic strength and pH on the binding of sanguinarine to deoxyribonucleic acid.
November 1985, Photochemistry and photobiology,
P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
Estrogen receptor activation precedes transformation. Effects of ionic strength, temperature, and molybdate.
August 1983, The Journal of biological chemistry,
P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
Effect of pH and ionic strength on the binding strength of anti-PF4/polyanion antibodies.
December 2017, European biophysics journal : EBJ,
P Blanchardie, and P Lustenberger, and J L Orsonneau, and M Denis, and S Bernard
pH and ionic strength dependence of protein (un)folding and ligand binding to bovine beta-lactoglobulins A and B.
December 2002, Biochemistry,
Copied contents to your clipboard!