-transparent.png){kind=logo}
Turnover of [14C]thiamin was studied in mice with Ehrlich ascites carcinoma fed a thiamin-deficient diet and injected with 5 or 2 micrograms/mouse of the labeled vitamin. The process of conversion of [14C]thiamin to thiamin pyrophosphate (TPP) was monitored by measuring the activities of transketolase, pyruvate dehydrogenase, and oxoglutarate dehydrogenase. The amount of coenzyme-unsaturated apotransketolase was assessed by measuring the TPP effect--determining transketolase activity with and without the addition of TPP in vitro. Tumor growth was accompanied by thiamin deficiency, manifested in an increase in [14C]thiamin incorporation into the host tissues and the absence of saturation of the tissues with the labeled vitamin over 13 days. Increased values for the turnover coefficients, reduction of thiamin-dependent enzyme activities, elevation of the TPP effect, and a decrease in urinary excretion of the radioactive products also provided evidence for a disturbance in thiamin metabolism. The severity of the disturbance in thiamin metabolism during malignant tumor growth was directly related to the dose of the exogenous vitamin.
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
December 1984,
Cancer letters,
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
May 1976,
The Journal of biological chemistry,
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
January 1981,
Voprosy pitaniia,
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
January 2011,
Bulletin of experimental biology and medicine,
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
January 1971,
Experientia,
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
November 2004,
Bulletin of experimental biology and medicine,
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
October 1969,
Strahlentherapie,
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
January 1992,
Journal of nutritional science and vitaminology,
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
June 1972,
Experientia,
R V Trebukhina, and
Y M Ostrovsky, and
V S Shapot, and
G N Mikhaltsevich, and
V N Tumanov
January 1982,
Cancer research,
