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The ability of Paf-acether to induce increased plasma protein extravasation (IPPE) was assessed in the skin of rats by measuring extravasation of 125I-bovine serum albumin. Paf-acether elicited dose-dependent IPPE with a threshold of 0.04 pmol and a maximum at 4.5 nmol, with an effective concentration (EC50) of 23 pmol. The EC50 of the 1-0-ester Paf-acether analog was 1.5 nmol whereas 2-lyso Paf-acether was ineffective in inducing IPPE. Serotonin and histamine were respectively 100 and 1,00-fold less potent than Paf-acether. High doses of Paf-acether also resulted in a dose related accumulation of III-indium oxine labeled platelets. Ultrastructural and radioisotopic studies showed that Paf-acether-induced IPPE was not dependent upon platelet accumulation. In addition, platelet release products were ruled out in this phenomenon, since a "cocktail" of indomethacin, methysergide and pyrilamine-maleate failed to significantly reduce Paf-acether-induced IPPE. Neutrophils were not involved in Paf-acether -induced IPPE since nitrogen mustard-treated animals presented with IPPE not significantly different from controls. Finally, the specificity of Paf-acether on IPPE formation was demonstrated by its complete prevention by prior intravenous injection of Paf-acether (6 ug/kg), but not of Paf-acether analogs, histamine or serotonin.
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
November 1982,
Thrombosis research,
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
January 1989,
Journal of lipid mediators,
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
March 1990,
Journal of leukocyte biology,
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
July 1989,
Journal of immunology (Baltimore, Md. : 1950),
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
January 1987,
Bulletin europeen de physiopathologie respiratoire,
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
March 1985,
Inflammation,
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
December 1991,
Lipids,
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
March 1993,
Lipids,
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
September 1991,
FEBS letters,
E Pirotzky, and
C P Page, and
R Roubin, and
A Pfister, and
W Paul, and
J Bonnet, and
J Benveniste
March 1982,
Thrombosis research,
