Biomaterial Database

Cryopreservation of human pulmonary alveolar macrophages. 1984

T L McLemore, and W G Johanson

Human pulmonary alveolar macrophages (PAM) from 30 patients with a variety of pulmonary diseases were tested for their ability to undergo cryopreservation. The cells were cryopreserved and stored under liquid nitrogen for intervals of 2, 4, 6, 8, or 12 wk, then assayed for viability (using the trypan dye exclusion method as well as the NADH-dependent cytochrome c reductase (cytochrome c) assay) and cellular function (as documented by the measurement of in vitro aryl hydrocarbon hydroxylase (AHH) induction by benzanthracene (BA) and by quantitation of PAM particulate phagocytosis). These results demonstrated no decrease in PAM viability after 2 wk of cryopreservation compared with that of the control cells (p greater than 0.30, paired, 2-tailed t test). However, PAM viability decreased slightly compared with the 0-time (p less than 0.003 in all instances) when cells were stored for intervals of 4, 6, 8, or 12 wk. In addition, cells demonstrated no further decrease in viability after being cryopreserved for as long as 12 wk, thawed, and cultured for as long as 48 h compared with the 0-time control cells (p greater than 0.10 in all instances). Similarly, when AHH induction was fluorometrically quantitated in PAM cultured for 24 h, there was no decrease in BA-induced AHH levels after a 2-wk cryopreservation period. However, AHH levels decreased slightly compared with those in the 0-time control cells (p less than 0.004), when PAM were stored for longer intervals of 4, 6, or 8 wk. The phagocytic activity of cryopreserved PAM was also quantitated using amorphous silica as a substrate.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D008264	Macrophages	The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)	Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D009245	NADH Dehydrogenase	A flavoprotein and iron sulfur-containing oxidoreductase that catalyzes the oxidation of NADH to NAD. In eukaryotes the enzyme can be found as a component of mitochondrial electron transport complex I. Under experimental conditions the enzyme can use CYTOCHROME C GROUP as the reducing cofactor. The enzyme was formerly listed as EC 1.6.2.1.	NADH Cytochrome c Reductase,Diaphorase (NADH Dehydrogenase),NADH (Acceptor) Oxidoreductase,NADH Cytochrome c Oxidoreductase,Dehydrogenase, NADH
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D011650	Pulmonary Alveoli	Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place.	Alveoli, Pulmonary,Alveolus, Pulmonary,Pulmonary Alveolus
D002470	Cell Survival	The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.	Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D005615	Freezing	Liquids transforming into solids by the removal of heat.	Melting
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001189	Aryl Hydrocarbon Hydroxylases	A large group of cytochrome P-450 (heme-thiolate) monooxygenases that complex with NAD(P)H-FLAVIN OXIDOREDUCTASE in numerous mixed-function oxidations of aromatic compounds. They catalyze hydroxylation of a broad spectrum of substrates and are important in the metabolism of steroids, drugs, and toxins such as PHENOBARBITAL, carcinogens, and insecticides.	Microsomal Monooxygenases,Xenobiotic Monooxygenases,Hydroxylases, Aryl Hydrocarbon,Monooxygenases, Microsomal,Monooxygenases, Xenobiotic
D001551	Benz(a)Anthracenes	Four fused benzyl rings with three linear and one angular, that can be viewed as a benzyl-phenanthrenes. Compare with NAPHTHACENES which are four linear rings.	Benz(b)Phenanthrenes,Naphthanthracenes
D014021	Tissue Preservation	The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).	Preservation, Tissue,Preservations, Tissue,Tissue Preservations