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The sensitivity of nuclei to micrococcal nuclease was compared in thyroid tissue obtained from euthyroid patients with solitary cold nodules and from patients with Graves' disease. A significant increase in the solubility and/or the sensitivity of nuclei to the nuclease was found in thyroid tissue from patients with Graves' disease. Electrophoretic analysis of DNA in chromatin solubilized by the nuclease revealed that the amount of oligonucleosomal DNA was increased, and that of polynucleosomal DNA was even more increased, in nuclei from Graves' thyroids than in those from normal thyroids. Polyacrylamide gel electrophoretic analysis in Triton acid-urea showed that the extent of histone acetylation in nuclei from Graves' thyroids was almost the same as in those from normal thyroids. These findings suggest that the state of chromatin organization in Graves' thyroid nuclei is different from that in normal thyroid nuclei and is independent of the extent of histone acetylation.
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
May 1983,
Biochemical and biophysical research communications,
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
June 1983,
Biochimica et biophysica acta,
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
October 1978,
The Journal of cell biology,
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
August 1978,
Biochemistry,
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
January 2006,
Methods in molecular biology (Clifton, N.J.),
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
January 1984,
Cell biology international reports,
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
June 1982,
Endocrinology,
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
October 1996,
Journal of biochemical and biophysical methods,
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
August 1980,
Science (New York, N.Y.),
M Inaba, and
N Hamada, and
H Morii, and
M Ohno, and
J Yamakawa, and
J No, and
T Mimura, and
K Ito, and
M Yukioka
January 1982,
Molekuliarnaia biologiia,
