Biomaterial Database

Extracellular calcium is involved in the mechanism of differentiation of mouse myeloid leukemia cells (M1) induced by 1 alpha, 25-dihydroxyvitamin D3. 1984

C Miyaura, and E Abe, and T Suda

We have reported that 1 alpha, 25-dihydroxyvitamin D3 [1 alpha, 25(OH)2D3] suppresses proliferation and induces differentiation of murine myeloid leukemia cells (M1) into macrophages. In the current study, M1 cells were cultured either with 2.0 or 0.15 mM total calcium to examine the effect of calcium on the process of differentiation induced by the vitamin. The 0.15 mM calcium medium greatly enhanced 1 alpha, 25-dihydroxyvitamin D3 [1 alpha, 25(OH)2D3]-induced inhibition of cell growth and suppression of [3H]thymidine incorporation. Addition of Verapamil, a calcium antagonist, to the 2.0 mM calcium medium also elicited similar responses. The absolute number of cells with phagocytic activity induced by 1 alpha, 25(OH)2D3 was almost identical in media containing either concentration of calcium, and in cultures with or without Verapamil. Culture in the 0.15 mM calcium medium or addition of Verapamil to the 2.0 mM calcium medium did not suppress cell growth nor induce phagocytic activity in the absence of the vitamin. To confirm the preferential effect of calcium on cell growth, M1 cells were pretreated for 3 days with 1 alpha, 25(OH)2D3 in either the 2.0 or 0.15 mM calcium medium. Then the pretreated cells were washed and subcultured in the absence of 1 alpha, 25(OH)2D3 in either medium. The growth rate was inhibited much more effectively in the subculture with 0.15 mM calcium than with 2.0 mM calcium. These results suggest that the M1 cells' increased requirement of extracellular calcium, caused by the treatment with 1 alpha, 25(OH)2D3, is closely related to cell growth rather than differentiation.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D007942	Leukemia, Experimental	Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.	Experimental Leukemia,Experimental Leukemias,Leukemia Model, Animal,Leukemias, Experimental,Animal Leukemia Model,Animal Leukemia Models,Leukemia Models, Animal
D010587	Phagocytosis	The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).	Phagocytoses
D002117	Calcitriol	The physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.	1 alpha,25-Dihydroxycholecalciferol,1 alpha,25-Dihydroxyvitamin D3,1, 25-(OH)2D3,1,25(OH)2D3,1,25-Dihydroxycholecalciferol,1,25-Dihydroxyvitamin D3,1 alpha, 25-dihydroxy-20-epi-Vitamin D3,1,25(OH)2-20epi-D3,1,25-dihydroxy-20-epi-Vitamin D3,20-epi-1alpha,25-dihydroxycholecaliferol,Bocatriol,Calcijex,Calcitriol KyraMed,Calcitriol-Nefro,Decostriol,MC-1288,MC1288,Osteotriol,Renatriol,Rocaltrol,Silkis,Sitriol,Soltriol,Tirocal,1 alpha,25 Dihydroxyvitamin D3,1,25 Dihydroxycholecalciferol,1,25 Dihydroxyvitamin D3,1,25 dihydroxy 20 epi Vitamin D3,Calcitriol Nefro,D3, 1 alpha,25-Dihydroxyvitamin,D3, 1,25-Dihydroxyvitamin,D3, 1,25-dihydroxy-20-epi-Vitamin,KyraMed, Calcitriol,MC 1288
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D004261	DNA Replication	The process by which a DNA molecule is duplicated.	Autonomous Replication,Replication, Autonomous,Autonomous Replications,DNA Replications,Replication, DNA,Replications, Autonomous,Replications, DNA
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia