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Using an inhibitory analysis, the different enzymatic nature of kininogenase and elastase activities in serine proteinase fractions (pI 8.3-10.75) isolated from human granulocyte lysates by isoelectrofocusing was demonstrated. The thermo- and acid-stable serine proteinase inhibitor from rabbit serum was shown to completely inhibit the kininogenase activity in these fractions but to have no inhibiting action on the elastase activity. On the contrast, the specific granulocyte elastase inhibitor, N-3-carbomethoxypropanoyl-L-alanyl-L-alanyl-L-prolyl-L-valyl-chloromethylketone , inhibits granulocyte elastase and does not inhibit the kininogenase activity in lysate fractions. The efficiency of granulocyte elastase inhibition by this chloromethylketone is evaluated by the kinetic parameters k3, Ki. The values of k3/Ki for granulocyte elastase forms with pI of 10.75, 8.9 and 8.0 are 1430, 670 and 360 M-1 S-1, respectively and show effective inhibition of the three forms by this inhibitor. Based on the different degree of inhibition of the three elastase forms by chloromethylketone inhibitor the existence of the family of elastaselike enzymes in human granulocytes is postulated.
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
April 1988,
The American review of respiratory disease,
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
September 1999,
Nihon rinsho. Japanese journal of clinical medicine,
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
January 1986,
FEBS letters,
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
February 1970,
Biochemical pharmacology,
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
January 1972,
The American review of respiratory disease,
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
December 1987,
Biochemistry international,
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
January 1988,
Biulleten' eksperimental'noi biologii i meditsiny,
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
November 1978,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
April 1981,
Chemical & pharmaceutical bulletin,
L V Platonova, and
O G Ogloblina, and
T S Paskhina, and
V F Martynov, and
E I Sorochinskaia
September 1999,
Die Pharmazie,
