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Treatment of nucleosomes with a low concentration of sodium dodecyl sulfate removed all proteins except histone H1 from DNA, thus confirming our previous observation on sheared chromatin. No redistribution of H1 occurred during this procedure for isolation of the H1-DNA complex. The H1-DNA complex was isolated from a nucleosome monomer, doubly labeled in its protein and DNA and fractionated according to the length of DNA, and then the distribution of H1 was analyzed quantitatively. The results indicated that the monomer consisted of two subspecies, one containing 160 base pairs of DNA and one molecule of H1, and the other containing 140 base pairs of DNA and no H1. Since no monomer with two molecules of H1 was found, it is concluded that the nucleosome core has a binding site for H1 on only one side, and thus that the nucleosome is not a dyad.
K Hayashi, and
T Hofstaetter, and
N Yakuwa
September 1979,
Nucleic acids research,
K Hayashi, and
T Hofstaetter, and
N Yakuwa
February 1976,
Nucleic acids research,
K Hayashi, and
T Hofstaetter, and
N Yakuwa
May 1993,
Proceedings of the National Academy of Sciences of the United States of America,
K Hayashi, and
T Hofstaetter, and
N Yakuwa
June 1979,
Canadian journal of biochemistry,
K Hayashi, and
T Hofstaetter, and
N Yakuwa
November 1977,
Proceedings of the National Academy of Sciences of the United States of America,
K Hayashi, and
T Hofstaetter, and
N Yakuwa
August 1993,
Journal of biomolecular structure & dynamics,
K Hayashi, and
T Hofstaetter, and
N Yakuwa
January 1978,
Doklady Akademii nauk SSSR,
K Hayashi, and
T Hofstaetter, and
N Yakuwa
January 1978,
Cold Spring Harbor symposia on quantitative biology,
K Hayashi, and
T Hofstaetter, and
N Yakuwa
October 2014,
Nature reviews. Molecular cell biology,
K Hayashi, and
T Hofstaetter, and
N Yakuwa
January 1978,
Doklady Akademii nauk SSSR,
