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We have investigated the kinetics of the loss of thymine dimers from the acid-insoluble fraction of several ultraviolet (UV)-irradiated cultured human cell lines. Our results show that UV fluences between 10 and 40 J/m2 produce an average of 21-85 x 10(5) thymine dimers per cell and an eventual maximal loss per cell of 12-20 x 10(5) thymine dimers. The time for half-maximal loss of dimers ranged from 12-22 h after UV irradiation. In contrast, the time for half-maximal repair synthesis of DNA measured by autoradiography was 4.5 h. This figure agrees well with reported half-maximal repair synthesis times, which range from 0.5 to 3.6 h based on our analysis. The discrepancy in the kinetics of the loss of thymine dimers from DNA and repair synthesis is discussed in terms of possible molecular mechanisms of thymine dimer excision in vivo and in terms of possible experimental artifacts.
U K Ehmann, and
K H Cook, and
E C Friedberg
November 1971,
Photochemistry and photobiology,
U K Ehmann, and
K H Cook, and
E C Friedberg
January 1975,
Basic life sciences,
U K Ehmann, and
K H Cook, and
E C Friedberg
June 1982,
Radiation research,
U K Ehmann, and
K H Cook, and
E C Friedberg
January 1974,
Folia microbiologica,
U K Ehmann, and
K H Cook, and
E C Friedberg
April 1965,
Radiation research,
U K Ehmann, and
K H Cook, and
E C Friedberg
June 1970,
Photochemistry and photobiology,
U K Ehmann, and
K H Cook, and
E C Friedberg
May 1976,
Journal of bacteriology,
U K Ehmann, and
K H Cook, and
E C Friedberg
July 1964,
Biochemical and biophysical research communications,
U K Ehmann, and
K H Cook, and
E C Friedberg
April 1975,
Biochimica et biophysica acta,
U K Ehmann, and
K H Cook, and
E C Friedberg
May 1987,
Mutation research,
