Biomaterial Database

Determination of the amino acid sequence of the C-terminal cyanogen bromide fragment of myoglobin from bottle nosed dolphin by mass spectrometric peptide mixture analysis. 1978

P Roepstorff

The C-terminal cyanogen bromide fragment of myoglobin from bottle nosed dolphin was digested with trypsin and two different derivatives of the digest were prepared, one being deuteroacetylated and deuteropermethylated, the other being submitted to one step of Edman degradation and then acetylated and permethylated. Comparison of the results obtained by computerized interpretation of the mass spectra of these two derivatives, combined with previous information about the sample, facilitated the sequence determination of all the peptides in the sample. Alignment of the peptides could then be carried out by homology with myoglobin from other cetaceans.

UI	MeSH Term	Description	Entries
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D009211	Myoglobin	A conjugated protein which is the oxygen-transporting pigment of muscle. It is made up of one globin polypeptide chain and one heme group.
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D003488	Cyanogen Bromide	Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.	Bromide, Cyanogen
D004289	Dolphins	Mammals of the families Delphinidae (ocean dolphins), Iniidae, Lipotidae, Pontoporiidae, and Platanistidae (all river dolphins). Among the most well-known species are the BOTTLE-NOSED DOLPHIN and the KILLER WHALE (a dolphin). The common name dolphin is applied to small cetaceans having a beaklike snout and a slender, streamlined body, whereas PORPOISES are small cetaceans with a blunt snout and rather stocky body. (From Walker's Mammals of the World, 5th ed, pp978-9)	Amazon Dolphins,Baiji,Cephalorhynchus,Chinese River Dolphin,Delphinidae,False Killer Whale,Franciscana,Fraser's Dolphin,Ganges Dolphin,Gray Grampus,Humpback Dolphins,Irrawaddy River Dolphin,La Plata Dolphin,Lagenorhynchus,Lissodelphis,Many-Toothed Blackfish,Marine Dolphins,Melon-Headed Whale,Ocean Dolphins,Piebald Dolphins,Platanista,Pontoporia blainvillei,Pygmy Killer Whale,Right Whale Dolphins,Risso's Dolphin,Rough-Toothed Dolphin,Sousa,Susus,Tucuxi Dolphin,Whale, False Killer,Whale, Melon-Headed,Whale, Pygmy Killer,White-Beaked Dolphins,Amazon River Dolphins,Atlantic White-Sided Dolphins,Feresa attenuata,Ganges River Dolphin,Grampus griseus,Inia geoffrensis,Lagenodelphis hosei,Lagenorhynchus acutus,Lagenorhynchus albirostris,Langenorhynchus obliquidens,Lipotes vexillifer,Orcaella brevirostris,Pacific White-Sided Dolphins,Peponocephala electra,Planista gangetica,Pseudorca crassidens,Sotalia fluviatilis,Steno bredanensis,Yangtze River Dolphin,Atlantic White Sided Dolphins,Atlantic White-Sided Dolphin,Baijus,Dolphin,Dolphin, Fraser's,False Killer Whales,Franciscanas,Fraser Dolphin,Frasers Dolphin,Humpback Dolphin,Lagenodelphis hoseus,Lagenorhynchus albirostri,Langenorhynchus obliquiden,Lissodelphi,Many Toothed Blackfish,Marine Dolphin,Melon Headed Whale,Melon-Headed Whales,Ocean Dolphin,Orcaella brevirostri,Pacific White Sided Dolphins,Pacific White-Sided Dolphin,Piebald Dolphin,Planista gangeticas,Platanistas,Pontoporia blainvilleus,Pseudorca crassiden,Pygmy Killer Whales,Right Whale Dolphin,Risso Dolphin,Rissos Dolphin,Rough Toothed Dolphin,Rough-Toothed Dolphins,Sotalia fluviatili,Tucuxi Dolphins,White Beaked Dolphins,White-Beaked Dolphin
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013058	Mass Spectrometry	An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.	Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass