-transparent.png){kind=logo}
Inactive kallikrein was purified from rat urine by a procedure including ammonium sulfate fractionation, DEAE cellulose chromatography, phenyl-Sepharose CL-4B chromatography, and gel filtration on Sephadex G-100 and Sephadex G-75 columns. The resulting preparation was essentially homogeneous, as assessed by polyacrylamide gel electrophoresis. This preparation migrated as a single protein band on a SDS-polyacrylamide gel and the molecular weight was 41000. The purified material underwent marked activation by trypsin, but not by deoxycholate, Triton X-100, SDS or acidification. These results indicate that the purified inactive kallikrein is the precursor rather than a complex with a substance binding to the active form of kallikrein.
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
November 1975,
FEBS letters,
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
June 1986,
Proceedings of the National Academy of Sciences of the United States of America,
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
November 1994,
Biochemical Society transactions,
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
October 1980,
The Journal of biological chemistry,
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
March 1981,
Journal of immunology (Baltimore, Md. : 1950),
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
October 1983,
The Journal of biological chemistry,
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
May 1992,
The Journal of biological chemistry,
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
January 1986,
NIDA research monograph,
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
March 1994,
Journal of lipid research,
M Takaoka, and
H Okamura, and
T Iwamoto, and
C Ikemoto, and
Y Mimura, and
S Morimoto
April 1984,
Biochemical and biophysical research communications,
