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Under standard conditions, in the presence of GTP, highly purified eIF-2 and Co-eIF-2 factor preparations efficiently stimulated AUG-codon dependent but not physiological mRNA-dependent Met-tRNAf binding to 40S ribosomes. Replacement of GTP by a nonhydrolyzable GTP analog, GMP-PNP, in the above system, gave significant stimulation of Met-tRNAf binding to 40S ribosomes dependent on physiological mRNAs. Lower but significant stimulation of Met-tRNAf binding to 40S ribosomes was also observed when GTP was used in the presence of nucleoside 5'-diphosphate kinase (NDK) and ATP. ATP alone in the absence of NDK had no significant effect. This is the first report on the formation of a stable Met-tRNAf . 40S initiation complex dependent on physiological mRNAs and the factor requirements for such complex formation.
R Roy, and
N Nasrin, and
M F Ahmad, and
N K Gupta
July 1975,
Biochemical and biophysical research communications,
R Roy, and
N Nasrin, and
M F Ahmad, and
N K Gupta
September 1977,
Biochemical and biophysical research communications,
R Roy, and
N Nasrin, and
M F Ahmad, and
N K Gupta
October 1977,
Biochemical and biophysical research communications,
R Roy, and
N Nasrin, and
M F Ahmad, and
N K Gupta
April 1981,
Biochemical and biophysical research communications,
R Roy, and
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N K Gupta
February 1975,
The Journal of biological chemistry,
R Roy, and
N Nasrin, and
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N K Gupta
August 1980,
The Journal of biological chemistry,
R Roy, and
N Nasrin, and
M F Ahmad, and
N K Gupta
October 1976,
The Journal of biological chemistry,
R Roy, and
N Nasrin, and
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N K Gupta
May 1971,
Biochemical and biophysical research communications,
R Roy, and
N Nasrin, and
M F Ahmad, and
N K Gupta
June 1978,
Biochemical and biophysical research communications,
R Roy, and
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N K Gupta
September 1978,
The Journal of biological chemistry,
