Both agents caused loss of ruffled membrane and microtubules. Ruffled membrane decreased with time after treatment with vinblastine (4 mg/kg) and almost disappeared 8 h after treatment with either colchicine (4 mg/kg) or vinblastine (4 mg/kg). In vinblastine-treated animals, the number of microtubules were estimated in cross-sections of two regions of both smooth-ended and ruffle-ended ameloblasts; one region was distal and the other juxta-nuclear. In smooth-ended ameloblasts, microtubules of both the distal and juxta-nuclear cytoplasm almost disappeared after 2 h of treatment. In ruffle-ended ameloblasts, microtubules decreased in distal cytoplasm between 2 and 8 h; microtubules in the juxta-nuclear cytoplasm disappeared after 2 h. Both agents caused structural alterations and dislocation of cell organelles. The Golgi apparatus, sometimes separated into two parts, moved towards the proximal cytoplasm and an unusual accumulation of small vesicles was found in the proximal cytoplasm. These findings suggest that formation of ruffled membranes is dependent on microtubular function.