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Assay of phenol sulphotransferase in human blood. 1983

E J Pennings, and G M Van Kempen

An assay of phenol sulphotransferase in human blood with 4-hydroxy-3-methoxyphenylethylene glycol as partial substrate is described. The pH optimum with this substrate is 8.0. To demonstrate the heterogeneity of the enzyme in blood an alternative partial substrate, 4-methylumbelliferone, at an optimal pH of 7.4, is used. Enzyme activity is linear with time up to at least 60 minutes of incubation. With 4-hydroxy-3-methoxyphenylethylene glycol as the substrate a linear relation between phenol sulphotransferase activity and enzyme concentration exists. Phenol sulphotransferase activity shows no correlation with age or sex of healthy subjects. Varying ratios of the enzyme activities toward both substrates support the idea of two phenol sulphotransferases in blood. As nevertheless a significant correlation exists between both sulphotransferase activities we conclude that neither substrate is sulphated exclusively by one of the two enzymes. Because preparative procedures for platelets may lead to considerable losses of specific subpopulations with relatively low or high enzyme activity, one of the advantages of using whole blood is that the activity in the whole platelet population is determined.

UI MeSH Term Description Entries
D008734 Methoxyhydroxyphenylglycol Synthesized from endogenous epinephrine and norepinephrine in vivo. It is found in brain, blood, CSF, and urine, where its concentrations are used to measure catecholamine turnover. Hydroxymethoxyphenylglycol,MHPG,MOPEG,Vanylglycol,4-Hydroxy-3-methoxyphenylethylene Glycol,4-Hydroxy-3-methoxyphenylethyleneglycol,4-Hydroxy-3-methoxyphenylglycol,Methoxyhydroxyphenylglycol, (+)-Isomer,Methoxyhydroxyphenylglycol, (+-)-Isomer,Methoxyhydroxyphenylglycol, (-)-Isomer,4 Hydroxy 3 methoxyphenylethylene Glycol,4 Hydroxy 3 methoxyphenylethyleneglycol,4 Hydroxy 3 methoxyphenylglycol
D001792 Blood Platelets Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation. Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006923 Hymecromone A coumarin derivative possessing properties as a spasmolytic, choleretic and light-protective agent. It is also used in ANALYTICAL CHEMISTRY TECHNIQUES for the determination of NITRIC ACID. Imecromone,Methylumbelliferone,Resocyanine,4-Methylumbelliferone,7-Hydroxy-4-methyl-coumarin,Cholestil,Mendiaxon,4 Methylumbelliferone,7 Hydroxy 4 methyl coumarin
D013466 Sulfurtransferases Enzymes which transfer sulfur atoms to various acceptor molecules. EC 2.8.1. Sulfurtransferase
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D015239 Arylsulfotransferase A sulfotransferase that catalyzes the sulfation of a phenol in the presence of 3'-phosphoadenylylsulfate as sulfate donor to yield an aryl sulfate and adenosine 3',5'-bisphosphate. A number of aromatic compounds can act as acceptors; however, organic hydroxylamines are not substrates. Sulfate conjugation by this enzyme is a major pathway for the biotransformation of phenolic and catechol drugs as well as neurotransmitters. EC 2.8.2.1. Phenol Sulfotransferase,Acetaminophen Sulphotransferase,Aryl Sulfotransferase,Catecholamine Sulfotransferase A,Catecholamine Sulfotransferase B,Dopamine Sulfotransferase,Flavonoid Sulfotransferase,Phenol Sulfokinase,Phenol Sulfotransferase M,Phenol Sulfotransferase P,Phenolsulfokinase,Phenolsulfotransferase P,Sulfokinase,Sulfotransferase A, Catecholamine,Sulfotransferase B, Catecholamine,Sulfotransferase M, Phenol,Sulfotransferase P, Phenol,Sulfotransferase, Aryl,Sulfotransferase, Dopamine,Sulfotransferase, Flavonoid,Sulfotransferase, Phenol,Sulphotransferase, Acetaminophen

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