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Colorimetric enzymatic measurement of serum total 3 alpha-hydroxy bile acid concentrations without extraction. 1984

M Y Qureshi, and S M Smith, and G M Murphy

A new method for the simpler colorimetric assay of serum total 3 alpha-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 mumol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 +/- 5.1 mumol/l (mean +/- SD) and in 20 normal plasma randomly collected were 4.9 +/- 4.2 mumol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use.

UI MeSH Term Description Entries
D009580 Nitroblue Tetrazolium Colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease. Nitro-BT,Nitrotetrazolium Blue,Tetrazolium Nitroblue,Blue, Nitrotetrazolium,Nitroblue, Tetrazolium,Tetrazolium, Nitroblue
D011933 Reagent Kits, Diagnostic Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use. Diagnostic Reagent Kits,Diagnostic Reagents and Test Kits,Diagnostic Test Kits,In Vitro Diagnostic Device,In Vitro Diagnostic Devices,In Vitro Diagnostic Medical Device,In Vitro Diagnostic Medical Devices,Kits, Diagnostic Reagent,Diagnostic Reagent Kit,Diagnostic Test Kit,Kit, Diagnostic Reagent,Kit, Diagnostic Test,Kits, Diagnostic Test,Reagent Kit, Diagnostic,Test Kit, Diagnostic,Test Kits, Diagnostic
D003116 Color The visually perceived property of objects created by absorption or reflection of specific wavelengths of light. Colors
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001647 Bile Acids and Salts Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones. Bile Acid,Bile Salt,Bile Salts,Bile Acids,Acid, Bile,Acids, Bile,Salt, Bile,Salts, Bile
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013256 Steroids A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain vitamins, bile acids, alcohols (STEROLS), and certain natural drugs and poisons. Steroids have a common nucleus, a fused, reduced 17-carbon atom ring system, cyclopentanoperhydrophenanthrene. Most steroids also have two methyl groups and an aliphatic side-chain attached to the nucleus. (From Hawley's Condensed Chemical Dictionary, 11th ed) Steroid,Catatoxic Steroids,Steroids, Catatoxic
D015096 3-Hydroxysteroid Dehydrogenases Catalyze the oxidation of 3-hydroxysteroids to 3-ketosteroids. 3-beta-Hydroxysteroid Dehydrogenase,3 Hydroxysteroid Dehydrogenases,3 beta Hydroxysteroid Dehydrogenase,Dehydrogenase, 3-beta-Hydroxysteroid,Dehydrogenases, 3 Hydroxysteroid,Dehydrogenases, 3-Hydroxysteroid,Hydroxysteroid Dehydrogenases, 3
D045002 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) A 3-hydroxysteroid dehydrogenase which catalyzes the reversible reduction of the active androgen, DIHYDROTESTOSTERONE to 5 ALPHA-ANDROSTANE-3 ALPHA,17 BETA-DIOL. It also has activity towards other 3-alpha-hydroxysteroids and on 9-, 11- and 15- hydroxyprostaglandins. The enzyme is B-specific in reference to the orientation of reduced NAD or NADPH. 3 alpha-Hydroxysteroid Dehydrogenase,3 alpha-Hydroxysteroid Dehydrogenase (B-Specific),3 alpha-Hydroxysteroid Oxidoreductase,3alpha-Hydroxysteroid Dehydrogenase (B-specific),5 alpha-3 alpha-Hydroxysteroid Dehydrogenase,5 alpha-Dihydroprogesterone 3 alpha-Hydroxysteroid Oxidoreductase,5-DP-3 alpha-HSO,NADPH-5 alpha-Dihydroprogesterone 3 alpha-Hydroxysteroid Oxidoreductase,3 alpha Hydroxysteroid Dehydrogenase,3 alpha Hydroxysteroid Oxidoreductase,5 alpha Dihydroprogesterone 3 alpha Hydroxysteroid Oxidoreductase,Dehydrogenase, 3 alpha-Hydroxysteroid,NADPH 5 alpha Dihydroprogesterone 3 alpha Hydroxysteroid Oxidoreductase,Oxidoreductase, 3 alpha-Hydroxysteroid

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