BIOMAT Database Logo BIOMAT Database Logo

Isolation and identification of enkephalins in pedal ganglia of Mytilus edulis (Mollusca). 1984

M K Leung, and G B Stefano

An acid extract of pedal ganglia of the mollusc Mytilus edulis was fractionated by high-pressure liquid chromatography with a reverse-phase column. Peak fractions with retention times of those of [Met]- and [Leu]enkephalin were subjected to binding assays in both invertebrate and vertebrate tissues. The results showed that these fractions have the same binding activities as authentic enkephalins. Peptides from these fractions were purified by high-pressure liquid chromatography under isocratic conditions. Sequential amino acid analyses showed that these peptides have the same primary structures as [Met]- and [Leu]enkephalin. These results with M. edulis suggest that invertebrates possess an enkephalinergic system similar to that of higher organisms.

UI MeSH Term Description Entries
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004743 Enkephalin, Leucine One of the endogenous pentapeptides with morphine-like activity. It differs from MET-ENKEPHALIN in the LEUCINE at position 5. Its first four amino acid sequence is identical to the tetrapeptide sequence at the N-terminal of BETA-ENDORPHIN. Leucine Enkephalin,5-Leucine Enkephalin,Leu(5)-Enkephalin,Leu-Enkephalin,5 Leucine Enkephalin,Enkephalin, 5-Leucine,Leu Enkephalin
D004744 Enkephalin, Methionine One of the endogenous pentapeptides with morphine-like activity. It differs from LEU-ENKEPHALIN by the amino acid METHIONINE in position 5. Its first four amino acid sequence is identical to the tetrapeptide sequence at the N-terminal of BETA-ENDORPHIN. Methionine Enkephalin,5-Methionine Enkephalin,Met(5)-Enkephalin,Met-Enkephalin,5 Methionine Enkephalin,Enkephalin, 5-Methionine,Met Enkephalin
D004745 Enkephalins One of the three major families of endogenous opioid peptides. The enkephalins are pentapeptides that are widespread in the central and peripheral nervous systems and in the adrenal medulla. Enkephalin
D005724 Ganglia Clusters of multipolar neurons surrounded by a capsule of loosely organized CONNECTIVE TISSUE located outside the CENTRAL NERVOUS SYSTEM.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001665 Binding Sites The parts of a macromolecule that directly participate in its specific combination with another molecule. Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D049872 Bivalvia A class in the phylum MOLLUSCA comprised of mussels; clams; OYSTERS; COCKLES; and SCALLOPS. They are characterized by a bilaterally symmetrical hinged shell and a muscular foot used for burrowing and anchoring. Mussels,Bivalves,Clams,Bivalve,Bivalvias,Clam,Mussel

Related Publications

M K Leung, and G B Stefano
Mytilus edulis pedal ganglia express mu opiate receptor transcripts exhibiting high sequence identity with human neuronal mu1.
December 1999, Brain research. Molecular brain research,
M K Leung, and G B Stefano
Catch-relaxing peptide isolated from Mytilus pedal ganglia.
October 1987, Brain research,
M K Leung, and G B Stefano
Effects of cold stress on morphine-induced nitric oxide production and mu-opiate receptor gene expression in Mytilus edulis pedal ganglia.
January 2003, Neuro endocrinology letters,
M K Leung, and G B Stefano
Identification of insulin-like peptides in cerebral ganglia neurosecretory cells of the mussel Mytilus edulis.
December 1994, Tissue & cell,
M K Leung, and G B Stefano
Isolation and characterization of Mytilus edulis metallothionein genes.
January 2006, Comparative biochemistry and physiology. Toxicology & pharmacology : CBP,
M K Leung, and G B Stefano
Subcellular localization of serotonin-immunoreactivity in the pedal ganglion of Mytilus galloprovincialis (Mollusca, Bivalvia).
January 1988, Journal of submicroscopic cytology and pathology,
M K Leung, and G B Stefano
Characterization of the dopamine stimulated adenylate cyclase in the pedal ganglia of Mytilus edulis: interactions with etorphine, beta-endorphin, DALA, and methionine enkephalin.
March 1981, Cellular and molecular neurobiology,
M K Leung, and G B Stefano
Cold stress alters Mytilus edulis pedal ganglia expression of mu opiate receptor transcripts determined by real-time RT-PCR and morphine levels.
February 2002, Brain research. Molecular brain research,
M K Leung, and G B Stefano
Cytology and quantitative cytochemistry of a poliferative atypical hemocytic condition in Mytilus edulis (Bivalvia, mollusca).
June 1978, Journal of the National Cancer Institute,
M K Leung, and G B Stefano
Evidence for the presynaptic localization of a high affinity opiate binding site on dopamine neurons in the pedal ganglia of Mytilus edulis (Bivalvia).
September 1982, The Journal of pharmacology and experimental therapeutics,
Copied contents to your clipboard!