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A rapid and very sensitive enzymatic assay of estrogens was developed using the transhydrogenase reaction of the human estradiol-17 beta dehydrogenase and bioluminescence for the quantification of NADH accumulated during the first reaction. The assay requires two steps: first, the addition of transhydrogenase buffer, and a few minutes later the addition of the bioluminescence reagent. The method can determine 0.1 to 50 pg of estrogens in the assay, in 1 h, with a coefficient of variation of 5%.
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A M Boussioux, and
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A M Boularan, and
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A M Boularan, and
B Descomps, and
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American journal of clinical pathology,
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A M Boussioux, and
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Cancer research,
J C Nicolas, and
A M Boussioux, and
A M Boularan, and
B Descomps, and
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Journal of steroid biochemistry,
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A M Boussioux, and
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Bollettino della Societa italiana di biologia sperimentale,
