BIOMAT Database Logo BIOMAT Database Logo

Assignment of the human MARS gene, encoding methioninyl-tRNA synthetase, to chromosome 12 using human X Chinese hamster cell hybrids. 1984

R E Cirullo, and J J Wasmuth

We have isolated interspecific somatic cell hybrids between a temperature-sensitive Chinese hamster ovary (CHO) cell methioninyl -tRNA synthetase mutant and human peripheral leukocytes. The hybrids were selected at 39 degrees C which requires the retention and expression of the human gene, MARS , which complements the defective CHO gene. In vitro heat-inactivation experiments on the methioninyl -tRNA synthetase activity in cell-free extracts from heat-resistant hybrids indicate that the human form of this enzyme and, therefore, the human MARS gene is present in hybrid cells. Cytogenetic analysis of three independent temperature-resistant hybrids revealed the presence of a single human chromosome, number 12. Two other independent hybrids examined contained human chromosome 12 as well as a second human chromosome. Electrophoretic analysis of extracts from hybrid cell lines for a human chromosome 12 marker isozyme, LDH-B, showed a pattern of heterotetrameric bands consistent with the presence of the human form of this enzyme in these cells. The correlation between the presence of the human form of methioninyl -tRNA synthetase and human chromosome 12 in temperature-resistant hybrids indicates that the human MARS locus is located on this chromosome.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D007621 Karyotyping Mapping of the KARYOTYPE of a cell. Karyotype Analysis Methods,Analysis Method, Karyotype,Analysis Methods, Karyotype,Karyotype Analysis Method,Karyotypings,Method, Karyotype Analysis,Methods, Karyotype Analysis
D007770 L-Lactate Dehydrogenase A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist. Lactate Dehydrogenase,Dehydrogenase, L-Lactate,Dehydrogenase, Lactate,L Lactate Dehydrogenase
D008718 Methionine-tRNA Ligase An enzyme that activates methionine with its specific transfer RNA. EC 6.1.1.10. Methionyl T RNA Synthetase,Met-tRNA Ligase,Methionyl-tRNA Synthetase,Ligase, Met-tRNA,Ligase, Methionine-tRNA,Met tRNA Ligase,Methionine tRNA Ligase,Methionyl tRNA Synthetase,Synthetase, Methionyl-tRNA
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D010053 Ovary The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE. Ovaries
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002871 Chromosome Banding Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping. Banding, Chromosome,Bandings, Chromosome,Chromosome Bandings
D002874 Chromosome Mapping Any method used for determining the location of and relative distances between genes on a chromosome. Gene Mapping,Linkage Mapping,Genome Mapping,Chromosome Mappings,Gene Mappings,Genome Mappings,Linkage Mappings,Mapping, Chromosome,Mapping, Gene,Mapping, Genome,Mapping, Linkage,Mappings, Chromosome,Mappings, Gene,Mappings, Genome,Mappings, Linkage

Related Publications

R E Cirullo, and J J Wasmuth
Assignment of a human gene for tryptophanyl-tRNA synthetase to chromosome 14 using human-mouse somatic cell hybrids.
July 1976, Somatic cell genetics,
R E Cirullo, and J J Wasmuth
Assignment of the human gene for tryptophanyl-tRNA synthetase using human-mouse somatic cell hybrids.
January 1976, Birth defects original article series,
R E Cirullo, and J J Wasmuth
Assignment of the human gene for tryptophanyl-tRNA synthetase using human-mouse somatic cell hybrids.
January 1976, Cytogenetics and cell genetics,
R E Cirullo, and J J Wasmuth
Assignment of the gene for cytoplasmic glutamate-oxaloacetate transaminase to mouse chromosome 19 using chinese hamster x mouse somatic cell hybrids.
November 1980, Somatic cell genetics,
R E Cirullo, and J J Wasmuth
Chromosome assignment of a human gene for argininosuccinate synthetase expression in Chinese hamsterxhuman somatic cell hybrids.
April 1977, Experimental cell research,
R E Cirullo, and J J Wasmuth
Assignment of PGM3 to the long arm of human chromosome 6. Studies using Chinese hamster X human cell hybrids containing a human 6/15 translocation.
January 1980, Cytogenetics and cell genetics,
R E Cirullo, and J J Wasmuth
Assignment of a human galactose-1-phosphate uridylyltransferase gene (GALT1) to chromosome 9 in human-Chinese hamster somatic cell hybrids.
January 1978, Cytogenetics and cell genetics,
R E Cirullo, and J J Wasmuth
Assignment of the human gene for galactose-1-phosphate uridyltransferase to chromosome 9: studies with Chinese hamster-human somatic cell hybrids.
December 1977, Proceedings of the National Academy of Sciences of the United States of America,
R E Cirullo, and J J Wasmuth
Assignment of the human tryptophanyl-tRNA synthetase gene (WARS) to chromosome 14q32.2 --> q32.32.
January 1996, Cytogenetics and cell genetics,
R E Cirullo, and J J Wasmuth
Localization of human gene loci using spontaneous chromosome rearrangements in human-Chinese hamster somatic cell hybrids.
September 1975, American journal of human genetics,
Copied contents to your clipboard!